Biological Institute, Faculty of Science, Nagoya University, Nagoya 464, Japan.
Plant Physiol. 1974 May;53(5):717-22. doi: 10.1104/pp.53.5.717.
The effects of exogenous redox cofactors and purine analogues on the activation of the NAD kinase by Pfr were examined. Addition of phenazine methosulfate, flavin mononucleotide, or methylene blue increased the activation of NAD kinase by red light in a partially purified preparation of phytochrome. Phenazine methosulfate and flavin mononucleotide do not absorb light in the red or far red region, so they do not act as light receptors in this activation. Thus they probably intervene in electron transfer between phytochrome and NAD kinase. Addition of kinetin with these compounds increased photopotentiation further. In the presence of phenazine methosulfate and kinetin, the activation of NAD kinase by red light was counteracted by illumination with far red light immediately after red light.The relation between the dose of illumination with red light and the degree of activation of NAD kinase was measured. Illumination for 5 minutes with 800 ergs cm(-2) sec(-1) of red light was sufficient to obtain maximal activation. In the dark, the activated state of NAD kinase was maintained for about 10 min and then rapidly lost. The time of preservation of the activated state was greatly shortened by illumination with far red light, indicating the participation of phytochrome.
研究了外源氧化还原辅助因子和嘌呤类似物对 Pfr 激活 NAD 激酶的影响。在部分纯化的光敏色素制剂中，添加吩嗪甲硫酸盐、黄素单核苷酸或亚甲基蓝可增加红光对 NAD 激酶的激活作用。吩嗪甲硫酸盐和黄素单核苷酸在红光或远红光区域不吸收光，因此它们在这种激活中不作为光受体。因此，它们可能参与了质体蓝素和 NAD 激酶之间的电子转移。在这些化合物中添加激动素可进一步增强光增效作用。在吩嗪甲硫酸盐和激动素存在下，红光照射后立即用远红光照射会抵消红光对 NAD 激酶的激活作用。测量了红光照射剂量与 NAD 激酶激活程度之间的关系。用 800 ergs cm(-2) sec(-1)的红光照射 5 分钟足以获得最大激活。在黑暗中，NAD 激酶的激活状态可维持约 10 分钟，然后迅速丧失。用远红光照射大大缩短了激活状态的保存时间，表明质体参与了这一过程。
