Department of Biology, University of California, San Diego, La Jolla, California 92037.
Plant Physiol. 1969 Mar;44(3):435-8. doi: 10.1104/pp.44.3.435.
Experiments in which chloroplasts were washed with tris and tricine buffers at different pH's indicated that the non-protonated (uncharged) form of tris was inhibitory to the Hill reaction while the protonated form of tris and the zwitterionic forms of tricine were non-inhibitory. Buffers analogous to tris and tricine gave similar results. Photoreduction of NADP could be restored to the inhibited chloroplasts by adding the reduced forms of p-hydroquinone, p-aminophenol, p-phenylenediamine, benzidine, semicarbazide, and dihydroxydiphenyl, all of which donated electrons to photosystem II. Photoreduction of ferricyanide was shown with those donor systems (benzidine and semicarbazide) which did not react chemically with ferricyanide. Photophosphorylation was also restored with all of the electron donors except semicarbazide.
实验表明，用 tris 和 tricine 缓冲液在不同 pH 值下洗涤叶绿体，非质子化（不带电荷）的 tris 形式对希尔反应有抑制作用，而质子化的 tris 形式和 tricine 的两性离子形式则没有抑制作用。类似 tris 和 tricine 的缓冲液也得到了类似的结果。用光系统 II 还原型 p-对苯二酚、对氨基酚、对苯二胺、联苯胺、氨基脲和二羟二苯还原受抑制的叶绿体，可恢复 NADP 的光还原。在用那些不与铁氰化物发生化学反应的供体系统（联苯胺和氨基脲）进行铁氰化物的光还原实验中，也观察到了铁氰化物的光还原。除了氨基脲之外，所有的电子供体都能恢复磷酸化。
