黄瓜幼苗中的多种胺氧化酶。
Multiple amine oxidases in cucumber seedlings.
作者信息
Percival F W, Purves W K
机构信息
Department of Biological Sciences, University of California, Santa Barbara, California 93106.
出版信息
Plant Physiol. 1974 Oct;54(4):601-7. doi: 10.1104/pp.54.4.601.
Cell-free extracts of cucumber (Cucumis sativus L. cv. National Pickling) seedlings were found to have amine oxidase activity when assayed with tryptamine as a substrate. Studies of the effect of lowered pH on the extract indicated that this activity was heterogeneous, and three amine oxidases could be separated by ion exchange chromatography. The partially purified enzymes were tested for their activities with several substrates and for their sensitivities to various amine oxidase inhibitors. One of the enzymes may be a monoamine oxidase, although it is inhibited by some diamine oxidase inhibitors. The other two enzymes have properties more characteristic of the diamine oxidases. The possible relationship of the amine oxidases to indoleacetic acid biosynthesis in cucumber seedlings is discussed.
以色胺为底物进行测定时，发现黄瓜（Cucumis sativus L. cv. National Pickling）幼苗的无细胞提取物具有胺氧化酶活性。对提取物进行降低pH值影响的研究表明，该活性是异质的，并且三种胺氧化酶可以通过离子交换色谱法分离。对部分纯化的酶进行了几种底物的活性测试以及对各种胺氧化酶抑制剂的敏感性测试。其中一种酶可能是单胺氧化酶，尽管它受到一些二胺氧化酶抑制剂的抑制。另外两种酶具有更典型的二胺氧化酶特性。讨论了胺氧化酶与黄瓜幼苗中吲哚乙酸生物合成的可能关系。
Zotero 插件