Kaul K, Sabharwal P S
T. H. Morgan School of Biological Sciences, University of Kentucky, Lexington, Kentucky 40506.
Plant Physiol. 1974 Oct;54(4):644-8. doi: 10.1104/pp.54.4.644.
Kinetin-induced changes in dry weight, soluble protein content, delta-aminolevulinic acid dehydratase activity, and chlorophyll content of two clones of Nicotiana tabacum L. callus were studied. Kinetin brought about a marked increase in the delta-aminolevulinic acid dehydratase activity of nongreen tissue just before induction of greening. The experimental data suggested a possible induction of specific chloroplast protein(s) during the kinetin-induced greening of nongreen tobacco tissue. Kinetin caused a decline in the delta-aminolevulinic acid dehydratase activity and chlorophyll content of the green callus used in the present study.
研究了激动素对烟草(Nicotiana tabacum L.)愈伤组织两个克隆的干重、可溶性蛋白质含量、δ-氨基乙酰丙酸脱水酶活性和叶绿素含量的影响。在非绿色组织绿化诱导前,激动素使非绿色组织的δ-氨基乙酰丙酸脱水酶活性显著增加。实验数据表明,在激动素诱导非绿色烟草组织绿化过程中,可能诱导了特定的叶绿体蛋白。在本研究中,激动素导致绿色愈伤组织的δ-氨基乙酰丙酸脱水酶活性和叶绿素含量下降。