pH值和生长素对燕麦胚芽鞘细胞氯离子吸收的影响。
Effect of pH and Auxin on Chloride Uptake into Avena Coleoptile Cells.
作者信息
Rubinstein B
机构信息
Department of Botany, University of Massachusetts, Amherst, Massachusetts 01002.
出版信息
Plant Physiol. 1974 Dec;54(6):835-9. doi: 10.1104/pp.54.6.835.
The effect of pH on (36)Cl(-) movement into coleoptile cells (Avena sativa L. cv. Garry) was investigated and compared with effects of indoleacetic acid. (36)Cl(-) uptake, but not efflux, is stimulated when coleoptile sections are placed in media adjusted to pH levels from 5 to 3 after a preincubation period at pH 6.5. The enhancement is seen within 2 minutes, is not correlated with growth, and is completely erased by respiratory inhibitors. In comparison to the acid-induced stimulation, the stimulatory effect of indoleacetic acid on (36)Cl(-) uptake is also not accompanied by accelerated efflux, and indoleacetic acid does not further stimulate (36)Cl(-) uptake into 1-millimeter sections beyond that seen at pH 3.5 without auxin.
研究了pH对（36）Cl⁻进入燕麦胚芽鞘细胞（燕麦品种Garry）的影响，并与吲哚乙酸的作用进行了比较。当胚芽鞘切段在pH 6.5预孵育一段时间后置于pH值从5调节到3的培养基中时，（36）Cl⁻的摄取受到刺激，但外排不受影响。这种增强在2分钟内即可观察到，与生长无关，并且可被呼吸抑制剂完全消除。与酸诱导的刺激相比，吲哚乙酸对（36）Cl⁻摄取的刺激作用也不会伴随外排加速，并且吲哚乙酸不会进一步刺激（36）Cl⁻进入1毫米切段的摄取，其摄取量不会超过在没有生长素的pH 3.5条件下所观察到的量。
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