Department of Biology, University of California, San Diego, La Jolla, California 92037.
Plant Physiol. 1975 Feb;55(2):421-6. doi: 10.1104/pp.55.2.421.
Irradiating the mycelium of Neurospora crassa with moderate intensities of blue light causes a reversible photoreduction of a b-type cytochrome. The action spectrum for the photoreduction of cytochrome b is very similar to the absorption spectrum of flavin pigments. Prolonged irradiation of the mycelium with strong blue light irreversibly bleaches flavin-like pigments and as these pigments are bleached the photoresponse of cytochrome b is lost. We conclude from these and other data that a flavin is the photoreceptor pigment for the photoreduction of cytochrome b. The close similarity between the action spectrum for the photoreduction of cytochrome b and action spectra for a number of physiological photoresponses suggests that this photoreceptor pigment controls a wide variety of photobiological processes in a wide diversity of organisms.
用中等强度的蓝光照射粗糙脉孢菌的菌丝体，会导致 b 型细胞色素可逆地光还原。细胞色素 b 的光还原作用光谱与黄素色素的吸收光谱非常相似。用强蓝光长时间照射菌丝体，会不可逆地漂白类黄素色素，随着这些色素的漂白，细胞色素 b 的光响应也会丧失。我们从这些和其他数据中得出结论，黄素是细胞色素 b 光还原的光受体色素。细胞色素 b 光还原作用光谱与许多生理光响应的作用光谱非常相似，这表明这种光受体色素控制着广泛的生物多样性的各种生物的多种光生物过程。
