粗糙脉孢菌分生孢子对外源细胞色素c的还原作用：超氧化物歧化酶和蓝光的影响
Reduction of exogenous cytochrome c by Neurospora crassa conidia: effects of superoxide dismutase and blue light.
作者信息
Takahama U, Shimizu-Takahama M, Egashira T
出版信息
J Bacteriol. 1982 Oct;152(1):151-6. doi: 10.1128/jb.152.1.151-156.1982.
The reduction of externally added cytochrome c by Neurospora crassa conidia was observed. The reduction was stimulated by antimycin A and suppressed partially by superoxide dismutase. When conidia were treated with diethyldithiocarbamate, which inactivated endogenous superoxide dismutase, the cytochrome c reduction was stimulated. Blue light also stimulated the cytochrome c reduction. Azide, which inhibits photochemical reactions mediated by flavins, suppressed the blue light effect. Superoxide dismutase partially suppressed the cytochrome c reduction in the light. The results suggest that O2(-) participates in the cytochrome c reduction by conidia and the flavins or flavoproteins are candidates for the receptor pigment of blue light to stimulate the cytochrome c reduction. It was also suggested that the redox component(s), which could directly transfer its reducing equivalents to exogenous cytochrome c, was present at the surface of conidia.
观察到粗糙脉孢菌分生孢子对外源添加的细胞色素c的还原作用。抗霉素A刺激了这种还原作用，而超氧化物歧化酶则部分抑制了该作用。当分生孢子用二乙基二硫代氨基甲酸盐处理时，内源性超氧化物歧化酶失活，细胞色素c的还原作用受到刺激。蓝光也刺激了细胞色素c的还原作用。叠氮化物抑制由黄素介导的光化学反应，它抑制了蓝光效应。超氧化物歧化酶在光照下部分抑制了细胞色素c的还原作用。结果表明，O2(-)参与了分生孢子对细胞色素c的还原作用，黄素或黄素蛋白是蓝光刺激细胞色素c还原作用的受体色素候选物。还表明，能够将其还原当量直接转移到外源细胞色素c的氧化还原成分存在于分生孢子表面。
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