Edgerton M, Cline M G, Rehm M M
Department of Botany, Ohio State University, Columbus, Ohio 43210.
Plant Physiol. 1975 Apr;55(4):695-8. doi: 10.1104/pp.55.4.695.
The effects of cyclic adenosine 3':5'-monophosphate (cAMP) on the growth of Avena coleoptile segments over 4 to 10 hours were monitored with a position sensing transducer. At pH 6, cAMP (0.1 mm with and without 2.5 mm glucose; or 2 mm alone) or dibutyryl cAMP (0.1 mm) was added at the beginning of the experiment, or after about 1 hour or after about 6 or 7 hours. Under all conditions tested, cAMP compounds had little or no effect on coleoptile segment elongation. Inasmuch as cAMP does not duplicate the rapid and vigorous elongation obtained with 2 mum auxin, the hypothesis that cAMP is a mediator of auxin activity is not supported by experimental evidence in this system. This conclusion is dependent upon the assumption that the cAMP compounds penetrated the tissue.
用位置传感换能器监测了环磷酸腺苷（cAMP）在4至10小时内对燕麦胚芽鞘切段生长的影响。在pH 6条件下，于实验开始时、约1小时后或约6或7小时后添加cAMP（0.1 mM，分别添加和不添加2.5 mM葡萄糖；或单独添加2 mM）或二丁酰环磷腺苷（0.1 mM）。在所有测试条件下，cAMP化合物对胚芽鞘切段伸长几乎没有影响。由于cAMP不能重现2 μM生长素所引起的快速而强烈的伸长，在该系统中，cAMP是生长素活性介质的假说未得到实验证据的支持。这一结论取决于cAMP化合物能穿透组织这一假设。
