Bamberger E S, Avron M
Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
Plant Physiol. 1975 Oct;56(4):481-5. doi: 10.1104/pp.56.4.481.
The sites of action of several compounds, reported to inhibit CO(2) fixation by chloroplast preparations were located by developing assays in lettuce chloroplasts to test their effect on partial reactions of the carbon cycle and on carbonic anhydrase. The results indicated that: d, l-glyceral-dehyde and 5'-AMP inhibit phosphoribulose kinase or isomerase. 3-Phosphoglyceric acid and 6-phosphogluconate inhibit ribulose diphosphate carboxylase. Azide, Mg(2+), and nitrite inhibit the activity of carbonic anhydrase of lettuce chloroplasts and light-dependent CO(2) fixation by intact chloroplasts with similar sensitivities. None of these inhibited CO(2) fixation in ruptured chloroplasts. It is suggested that the inhibition by azide, nitrite, and magnesium ions of CO(2) fixation by intact chloroplasts is due to their inhibition of the activity of carbonic anhydrase.
据报道,有几种化合物可抑制叶绿体制剂对二氧化碳的固定作用。通过在生菜叶绿体中开展分析实验,以测试它们对碳循环部分反应及碳酸酐酶的影响,从而确定了这些化合物的作用位点。结果表明:d,l-甘油醛和5'-AMP抑制磷酸核酮糖激酶或异构酶。3-磷酸甘油酸和6-磷酸葡萄糖酸抑制二磷酸核酮糖羧化酶。叠氮化物、镁离子和亚硝酸盐对生菜叶绿体碳酸酐酶的活性以及完整叶绿体的光依赖性二氧化碳固定作用具有相似的抑制敏感性。这些物质均不抑制破裂叶绿体中的二氧化碳固定作用。有人提出,叠氮化物、亚硝酸盐和镁离子对完整叶绿体二氧化碳固定作用的抑制是由于它们对碳酸酐酶活性的抑制。