海洋藻类中的碳酸酐酶。
Carbonic anhydrase in marine algae.
作者信息
Bowes G W
出版信息
Plant Physiol. 1969 May;44(5):726-32. doi: 10.1104/pp.44.5.726.
An electrometric system for determination of carbonic anhydrase activity was constructed. Enzyme activity was assayed in homogenates of marine macroscopic Chlorophyta, Rhodophyta, and Phaeophyta. Plants surveyed included Ulva expansa (Setchell) Setchell and Gardner, Codium fragile (Suringar) Hariot, Enteromorpha sp., Chaetomorpha torta (Farlow) McClatchie (Chlorophyta); Laurencia papillosa (Greville). Plocamium coccineum var. pacificum (Kylin) Dawson, Pterocladia capillacea (Gmelin) Bornet and Thuret. Gigartina armata J. Agardh (Rhodophyta); Eisenia arborea Areschoug and Macrocystis pyrifera (Linnaeus) C. A. Agardh (Phaeophyta). Activity was present in all algae; in the Phaeophyta this could be demonstrated only after dialysis. p-Chloromercuriphenylsulfonic acid (10(-4)m) decreased activity in 1 species, Plocamium; this inhibition could be almost completely overcome with the addition of 10(-3)m dithiothreitol. In 2 green and 2 red algae assayed for sensitivity to acetazolamide (Diamox), inhibition was complete at 10(-4)m concentration of inhibitor. Dithiothreitol at a concentration of 10(-3)m did not enhance activity in any of the homogenates, and was not necessary for enzyme expression.
构建了一种用于测定碳酸酐酶活性的测电系统。在海洋大型绿藻、红藻和褐藻的匀浆中测定酶活性。所检测的植物包括膨胀浒苔（Setchell）Setchell和Gardner、脆弱刚毛藻（Suringar）Hariot、浒苔属、扭曲硬毛藻（Farlow）McClatchie（绿藻门）；乳头劳伦藻（Greville）、太平洋海萝变种（Kylin）Dawson、鹿角沙菜（Gmelin）Bornet和Thuret、武装杉藻J. Agardh（红藻门）；树状艾氏藻Areschoug和巨藻（Linnaeus）C. A. Agardh（褐藻门）。所有藻类均有活性；在褐藻中，只有经过透析才能证明这一点。对氯汞苯磺酸（10⁻⁴m）使一种藻类——海萝的活性降低；加入10⁻³m二硫苏糖醇后，这种抑制作用几乎可以完全克服。在对2种绿藻和2种红藻进行乙酰唑胺（醋氮酰胺）敏感性检测时，抑制剂浓度为10⁻⁴m时抑制作用完全。浓度为10⁻³m的二硫苏糖醇在任何匀浆中均未增强活性，且酶表达也不需要它。
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