离体羽扇豆根瘤培养研究：I. 乙炔还原活性的维持和诱导。
Studies with detached lupin root nodules in culture: I. Maintenance and induction of acetylene reduction activity.
机构信息
Plant Physiology Division, Department of Scientific and Industrial Research, Palmerston North, New Zealand.
出版信息
Plant Physiol. 1975 Nov;56(5):665-70. doi: 10.1104/pp.56.5.665.
Abstract
A method has been developed for culturing detached nitrogen-fixing root nodules of lupin (Lupinus angustifolius L.) on a simple nutrient medium. Under the best conditions devised, the acetylene reduction activity of mature detached nodules was maintained at 10 to 25 nmoles of ethylene hr(-1) mg(-1) fresh weight for 3 days. Under the same culture conditions, immature nodules increased their acetylene reduction activity from 0.01 nmole or less to about 1 nmole hr(-1) mg(-1) fresh weight.
摘要
已经开发出一种在简单营养培养基上培养分离的羽扇豆（ Lupinus angustifolius L. ）固氮根瘤的方法。在设计的最佳条件下，成熟的分离根瘤的乙炔还原活性在 3 天内保持在 10 至 25nmoles 的乙烯 hr(-1)mg(-1)鲜重。在相同的培养条件下，未成熟的根瘤将其乙炔还原活性从 0.01nmole 或更少增加到约 1nmole hr(-1)mg(-1)鲜重。
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