Plant Physiology Division, Department of Scientific and Industrial Research, Private Bag, Palmerston North, New Zealand.
Plant Physiol. 1977 Nov;60(5):800-2. doi: 10.1104/pp.60.5.800.
Zone sedimentation through sucrose gradients was used for preparing Rhizobium bacteroids from lupin nodules and for separating them into slowly and rapidly sedimenting fractions.The purity of the bacteroids was established by electron microscopy and by enzyme assays, and they were shown to contain a CO-insensitive cytochrome c oxidase.Bacteroids sedimented more rapidly than broth-cultured Rhizobium bacteria, and bacteroids from old nodules sedimented more rapidly than bacteroids from young nodules.THERE APPEAR TO BE AT LEAST TWO FORMS OF BACTEROID IN OLD NODULES: slowly sedimenting bacteroids with moderate colony-forming ability resembling the bacteroids found in young nodules, and rapidly sedimenting bacteroids with much lower colony-forming ability.
蔗糖梯度区带离心法被用来从羽扇豆根瘤中制备根瘤菌细菌体，并将其分离为沉降速度较慢和较快的两个部分。细菌体的纯度通过电子显微镜和酶分析来确定，并且它们被证明含有一种对 CO 不敏感的细胞色素 c 氧化酶。细菌体的沉降速度快于培养液培养的根瘤菌细菌体，并且来自老根瘤的细菌体比来自年轻根瘤的细菌体沉降速度更快。在老根瘤中似乎至少有两种类型的细菌体：沉降速度较慢、具有中等菌落形成能力的细菌体，类似于在年轻根瘤中发现的细菌体，以及沉降速度较快、菌落形成能力低得多的细菌体。
