Faculty of Science, Department of Biophysics and Biochemistry, University of Tokyo, Hongo-Tokyo, Japan.
Plant Physiol. 1975 Dec;56(6):791-6. doi: 10.1104/pp.56.6.791.
The temperature dependence of the yield of chlorophyll a fluorescence was measured at room temperatures in living algal cells and higher plant chloroplasts. 3-(3',4'-Dichlorophenyl)-1, 1-dimethylurea was added to the samples during the measurements in order to eliminate the influence of photosynthetic photochemical reactions on the fluorescence yield.In Anacystis nidulans the maximum in the curve for the fluorescence yield versus temperature occurred near the temperatures at which the transition of physical phase of the membrane lipids changes from the liquid crystalline to the mixed solid-liquid crystalline states. These findings suggest that the occurrence of a fluorescence maximum in the temperature-dependence curve is an indication of the thermal transition of physical phase of membrane lipids. Similar but less distinct maxima were found in Cyanidium caldarium and Euglena gracilis. No maxima were seen in the curves for chlorophyll a fluorescence versus temperature in Chlorella pyrenoidosa, Plectonema boryanum, Stichococcus bacillaris Phaeodactylum tricornutum, or in chloroplasts of the higher plants, spinach, lettuce, tomato, and Tidestromia oblongifolia.The fluorescence yield of allophycocyanin in Anacystis did not show such a maximum, but did show a steep increase with decreasing temperature below 5 C. the fluorescence yields of chlorophyll a and phycobilins extracted from Anacystis increased monotonously with decreasing temperature.
在活体藻类细胞和高等植物叶绿体中，于室温下测量了叶绿素 a 荧光产量对温度的依赖关系。在测量过程中，向样品中添加 3-(3',4'-二氯苯基)-1,1-二甲基脲，以消除光合作用光化学反应对荧光产量的影响。在鱼腥藻中，荧光产量与温度关系曲线的最大值出现在膜脂物理相从液晶态向混合固-液晶态转变的温度附近。这些发现表明，荧光最大值出现在温度依赖性曲线中是膜脂物理相热转变的一个指示。在蓝藻和绿眼虫中也发现了类似但不太明显的最大值。在栅藻、束毛藻、聚球藻、三角褐指藻或高等植物叶绿体的叶绿素 a 荧光与温度关系曲线中没有看到最大值。鱼腥藻中别藻蓝蛋白的荧光产量没有出现这样的最大值，但在 5°C 以下，随着温度的降低，荧光产量急剧增加。从鱼腥藻中提取的叶绿素 a 和藻胆蛋白的荧光产量随着温度的降低单调增加。
