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从玉米中筛选二氯喹啉酸抗性光合作用突变体。

Selection of diquat resistance photosynthesis mutants from maize.

机构信息

Biological Sciences, University of Missouri, Columbia, Missouri 65201.

出版信息

Plant Physiol. 1976 Feb;57(2):284-5. doi: 10.1104/pp.57.2.284.

DOI:10.1104/pp.57.2.284
PMID:16659467
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC542008/
Abstract

Resistance of a seedling to the herbicide 1,1'-ethylene-2,2'-dipyridylium bromide (diquat) can be used as a selective technique for photosynthesis mutants in Zea mays L. Diquat requires reduction by the light reaction in order to kill leaf cells and, therefore, nonphotosynthetic mutants survive. This technique was tested using known mutants and is applicable to larger samples of plants than previous techniques. Resistance to diquat should allow selection of mutants on the oxidizing side of photosystem II which are not previously available in higher plants.

摘要

幼苗对除草剂 1,1'-乙烯-2,2'-联吡啶溴化物(敌草快)的抗性可用作玉米光合作用突变体的选择性技术。敌草快需要在光反应中被还原才能杀死叶细胞,因此非光合作用突变体得以存活。该技术已用于已知突变体的测试,并且比以前的技术更适用于更多的植物样本。对敌草快的抗性应允许选择在光合作用系统 II 的氧化侧的突变体,这些突变体在高等植物中以前是不可用的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2192/542008/eb5dd9680f21/plntphys00156-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2192/542008/eb5dd9680f21/plntphys00156-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2192/542008/eb5dd9680f21/plntphys00156-0163-a.jpg

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本文引用的文献

1
A Method for Producing, Selecting, and Isolating Photosynthetic Mutants of Euglena gracilis.高产、筛选和分离衣藻光合突变体的方法。
Plant Physiol. 1975 Jan;55(1):142-4. doi: 10.1104/pp.55.1.142.
2
Chloroplast Reactions of Photosynthetic Mutants in Zea mays.玉米光合突变体的叶绿体反应。
Plant Physiol. 1974 Apr;53(4):589-95. doi: 10.1104/pp.53.4.589.
3
Photosynthetic Studies on a Pea-mutant Deficient in Chlorophyll.对一种叶绿素缺乏豌豆突变体的光合研究
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Detecting mutants that have impaired photosynthesis by their increased level of fluorescence.通过荧光水平升高来检测光合作用受损的突变体。
Plant Physiol. 1967 Sep;42(9):1284-7. doi: 10.1104/pp.42.9.1284.
5
A screening technique for photosynthetic mutants in unicellular algae.一种用于单细胞藻类光合突变体的筛选技术。
Nature. 1960 Oct 22;188:339-40. doi: 10.1038/188339b0.
6
A spectroscopic analysis of low-fluorescent mutants of Chlamydomonas reinhardti blocked in their water-splitting oxygen-evolving apparatus.莱茵衣藻水裂解放氧装置受阻的低荧光突变体的光谱分析。
Biochim Biophys Acta. 1972 Sep 20;275(3):395-400. doi: 10.1016/0005-2728(72)90220-4.
7
Analysis of photosynthesis in green algae through mutation studies.通过突变研究分析绿藻中的光合作用。
Photophysiology. 1973;8:65-96. doi: 10.1016/b978-0-12-282608-5.50008-4.