Department of Agronomy, University of Kentucky, Lexington, Kentucky 40506.
Plant Physiol. 1976 Mar;57(3):458-9. doi: 10.1104/pp.57.3.458.
Levels of nitrate reductase activity (EC 1.9.6.1.) as high as 11 mumoles nitrite produced/hour gram fresh weight were found in barley (Hordeum vulgare cv. Compana) roots grown under low oxygen conditions. Roots of plants given identical treatment under sterile conditions did not develop the high levels of nitrate reductase activity. The results suggest that the buildup of particulate, reduced viologen-utilizing nitrate reductase reported in barley roots may be caused by bacterial contamination. The nitrate reductase activity in roots grown under low oxygen conditions was not specific for reduced nicotinamide adenine dinucleotide like the assimilatory nitrate reductase (EC 1.6.6.1.) normally found in aerated plant roots.
在低氧条件下生长的大麦(Hordeum vulgare cv. Compana)根中发现,硝酸还原酶活性(EC 1.9.6.1.)水平高达 11 微摩尔亚硝酸盐/小时/克鲜重。在无菌条件下接受相同处理的植物根不会产生高水平的硝酸还原酶活性。结果表明,在大麦根中报道的颗粒状、利用还原型维生素 K 的硝酸还原酶的积累可能是由细菌污染引起的。在低氧条件下生长的根中的硝酸还原酶活性不像通常在充气植物根中发现的同化硝酸还原酶(EC 1.6.6.1.)那样特异性地针对还原型烟酰胺腺嘌呤二核苷酸。