Department of Biochemistry, The University, S10 2TN, Sheffield, UK.
Planta. 1979 Jan;144(3):249-53. doi: 10.1007/BF00388766.
The intracellular distribution of the enzymes of nitrate and ammonia assimilation in apical cells of pea (Pisum sativum L.) roots is described. Nitrate reductase (EC 1.6.6.2) was found to have no organelle association, and is considered to be located in the cytosol or possibly loosely bound to the outside of an organelle. Nitrite reductase and glutamate synthase (EC 2.6.1.53) are plastid located, as is glutamine synthetase (EC 6.3.1.2) although this enzyme also has activity in the cytosol. Glutamate dehydrogenase (EC 1.4.1.3) was found only in the mitochondrion.
描述了豌豆（Pisum sativum L.）根顶细胞中硝酸盐和氨同化酶的细胞内分布。发现硝酸还原酶（EC 1.6.6.2）没有细胞器关联，被认为位于细胞质或可能松散地结合在细胞器的外部。亚硝酸还原酶和谷氨酸合酶（EC 2.6.1.53）位于质体中，谷氨酰胺合成酶（EC 6.3.1.2）也是如此，尽管这种酶在细胞质中也具有活性。谷氨酸脱氢酶（EC 1.4.1.3）仅在线粒体中发现。
