Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824.
Plant Physiol. 1976 Jun;57(6):846-9. doi: 10.1104/pp.57.6.846.
The glucolipids formed upon feeding (U-(14)C)glucose to embryos of Zea mays were partially characterized with respect to: (a) metabolic turnover, (b) acid lability, (c) phosphorus content, (d) chromatographic properties, and (e) hydrolysis products. The chloroform-methanol-soluble assimilated radioactivity was examined specifically for occurrence of a glycosylated prenol phosphate. With the extraction conditions used, no evidence was found for formation of a glucosylated prenol phosphate. Several, as yet unidentified, acid-labile glucolipids undergoing metabolic turnover were observed. Four diglycerides were characterized as hydrolysis products of a fraction that contained (14)C-glucose and phosphorus, and was subject to metabolic turnover. Examination of the 1-butanol-soluble glucolipids from pea (Pisum sativum) seedlings also demonstrated anionic glucolipids, evidencing metabolic turnover but none with the properties of glucosylated prenol phosphate.
喂食(U-(14)C)葡萄糖给玉米(Zea mays)胚胎形成的糖脂,从以下方面进行了部分特征描述:(a)代谢周转率、(b)酸不稳定性、(c)磷含量、(d)色谱性质和(e)水解产物。特别检查了氯仿-甲醇可溶的同化放射性物质是否存在糖基化的 prenol 磷酸盐。使用所采用的提取条件,没有发现形成糖基化 prenol 磷酸盐的证据。观察到几种酸不稳定的糖脂,其经历代谢周转。鉴定出四种二酰基甘油,作为含有(14)C-葡萄糖和磷的部分的水解产物,该部分经历代谢周转。对豌豆(Pisum sativum)幼苗的 1-丁醇可溶糖脂的检查也证明了阴离子糖脂,证明了代谢周转,但没有一种具有糖基化 prenol 磷酸盐的性质。