Solomos T, Laties G G
Department of Biology and Molecular Biology Institute, University of California, Los Angeles, California 90024.
Plant Physiol. 1976 Jul;58(1):47-50. doi: 10.1104/pp.58.1.47.
The effects of cyanide and ethylene, respectively, were studied on the respiration of a fully cyanide-sensitive tissue-the fresh pea, a slightly cyanide-sensitive tissue-the germinating pea seedling, and a cyanide-insensitive tissue-the cherimoya fruit. Cyanide inhibition of both fresh pea and pea seedling respiration was attended by a conventional Pasteur effect where fermentation was enhanced with an accumulation of lactate and ethanol and a change in the level of glycolytic intermediates indicative of the activation of phosphofructokinase and pyruvate kinase accompanied by a sharp decline in ATP level. In these tissues, ethylene had little or no effect on the respiration rate, or on the level of glycolytic intermediates or ATP. By contrast, ethylene as well as cyanide enhanced both respiration and aerobic glycolysis in cherimoya fruits with no buildup of lactate and ethanol and with an increase in the level of ATP. The data support the proposition that for ethylene to stimulate respiration the capacity for cyanide-resistant respiration must be present.
对氰化物完全敏感的组织——新鲜豌豆；对氰化物轻度敏感的组织——发芽的豌豆幼苗；对氰化物不敏感的组织——番荔枝果实。氰化物对新鲜豌豆和豌豆幼苗呼吸作用的抑制伴随着传统的巴斯德效应，即发酵增强，乳酸和乙醇积累，糖酵解中间产物水平发生变化，表明磷酸果糖激酶和丙酮酸激酶被激活，同时ATP水平急剧下降。在这些组织中，乙烯对呼吸速率、糖酵解中间产物水平或ATP几乎没有影响。相比之下，乙烯和氰化物都增强了番荔枝果实的呼吸作用和好氧糖酵解，没有乳酸和乙醇的积累，且ATP水平升高。这些数据支持了这样一种观点，即乙烯要刺激呼吸作用，必须具备抗氰呼吸的能力。
