利用硒半胱氨酸由菜豆的半胱氨酰-tRNA 合成酶。
Utilization of Selenocysteine by a Cysteinyl-tRNA Synthetase from Phaseolus aureus.
机构信息
Department of Biological Sciences, State University of New York, Binghamton, New York 13901.
出版信息
Plant Physiol. 1976 Sep;58(3):248-52. doi: 10.1104/pp.58.3.248.
Abstract
An l-cysteinyl-tRNA synthetase (EC 6.1.1.16) from Phaseolus aureus has been purified approximately 200-fold. The enzyme uses selenocysteine as substrate in the ATP-PPi exchange assay; other cysteine analogs were inactive. The molecular weight as determined by Sephadex G-200 column chromatography is about 61,000; sodium dodecyl sulfate and 8 m urea acrylamide gel electrophoresis indicate that the enzyme is a dimer consisting of two identical monomers of molecular weight 30,000. A method for the preparation of selenocysteine from selenocystine is described.
摘要
来自 Phaseolus aureus 的 l-半胱氨酰-tRNA 合成酶（EC 6.1.1.16）已被纯化约 200 倍。该酶在 ATP-PPi 交换测定中使用硒代半胱氨酸作为底物；其他半胱氨酸类似物没有活性。通过 Sephadex G-200 柱层析法测定的分子量约为 61,000；十二烷基硫酸钠和 8 m 尿素丙烯酰胺凝胶电泳表明，该酶是由分子量为 30,000 的两个相同单体组成的二聚体。描述了一种从硒代胱氨酸制备硒代半胱氨酸的方法。
相似文献
1
Utilization of Selenocysteine by a Cysteinyl-tRNA Synthetase from Phaseolus aureus.利用硒半胱氨酸由菜豆的半胱氨酰-tRNA 合成酶。
Plant Physiol. 1976 Sep;58(3):248-52. doi: 10.1104/pp.58.3.248.
2
Methionyl-tRNA Synthetase from Phaseolus aureus: Purification and Properties.来自 Phaseolus aureus 的甲硫氨酰-tRNA 合成酶：纯化与性质。
Plant Physiol. 1981 Feb;67(2):325-9. doi: 10.1104/pp.67.2.325.
3
Cysteinyl-tRNA Synthetase from Phaseolus aureus: Purification and Properties.半胱氨酰-tRNA 合成酶（Cysteinyl-tRNA Synthetase）来自 Phaseolus aureus：纯化和性质。
Plant Physiol. 1977 Nov;60(5):670-4. doi: 10.1104/pp.60.5.670.
4
Reduction of DL-selenocystine and isolation of L-seleoncysteine.DL-硒代胱氨酸的还原及L-硒代半胱氨酸的分离
J Inorg Biochem. 1980 Jul;12(4):343-51. doi: 10.1016/s0162-0134(00)80275-5.
5
Cysteinyl-tRNA Synthetase from Astragalus Species.来自黄芪属植物的半胱氨酰 - tRNA合成酶。
Plant Physiol. 1979 Jun;63(6):1095-7. doi: 10.1104/pp.63.6.1095.
6
Sulphur metabolism in Paracoccus denitrificans. Purification, properties and regulation of cysteinyl-and methionyl-tRNA synthetase.反硝化副球菌中的硫代谢。半胱氨酰-tRNA合成酶和甲硫氨酰-tRNA合成酶的纯化、性质及调控
Biochim Biophys Acta. 1977 Mar 15;481(1):266-78. doi: 10.1016/0005-2744(77)90158-9.
7
Purification and some properties of rat liver tyrosyl-tRNA synthetase.大鼠肝脏酪氨酸-tRNA合成酶的纯化及某些性质
Biochim Biophys Acta. 1978 Oct 12;526(2):626-34. doi: 10.1016/0005-2744(78)90153-5.
8
Multiple molecular forms of cysteinyl-tRNA synthetase from rat liver: purification and subunit structure.大鼠肝脏中半胱氨酰 - tRNA合成酶的多种分子形式：纯化及亚基结构
Biochim Biophys Acta. 1976 Nov 8;452(1):271-83. doi: 10.1016/0005-2744(76)90080-2.
9
Purification and properties of chloroplast leucyl-tRNA synthetase from a higher plant: Phaseolus vulgaris.高等植物菜豆叶绿体亮氨酰-tRNA合成酶的纯化及性质
J Biol Chem. 1982 Aug 25;257(16):9598-604.
10
Enzymatic characterisation of the multifunctional enzyme delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Streptomyces clavuligerus.来自棒状链霉菌的多功能酶δ-（L-α-氨基己二酰基）-L-半胱氨酰-D-缬氨酸合成酶的酶学特性分析
Eur J Biochem. 1992 Apr 15;205(2):687-94. doi: 10.1111/j.1432-1033.1992.tb16830.x.
引用本文的文献
1
A cysteinyl-tRNA synthetase variant confers resistance against selenite toxicity and decreases selenocysteine misincorporation.半胱氨酰-tRNA 合成酶变体赋予对亚硒酸盐毒性的抗性，并减少硒代半胱氨酸错掺入。
J Biol Chem. 2019 Aug 23;294(34):12855-12865. doi: 10.1074/jbc.RA119.008219. Epub 2019 Jul 11.
2
Plant perception of β-aminobutyric acid is mediated by an aspartyl-tRNA synthetase.植物对 β-氨基丁酸的感知是由天冬氨酰-tRNA 合成酶介导的。
Nat Chem Biol. 2014 Jun;10(6):450-6. doi: 10.1038/nchembio.1520. Epub 2014 Apr 28.
3
Amino acid modifications on tRNA.转运核糖核酸（tRNA）上的氨基酸修饰
Acta Biochim Biophys Sin (Shanghai). 2008 Jul;40(7):539-53. doi: 10.1111/j.1745-7270.2008.00435.x.
4
From one amino acid to another: tRNA-dependent amino acid biosynthesis.从一种氨基酸到另一种氨基酸：依赖于转运RNA的氨基酸生物合成
Nucleic Acids Res. 2008 Apr;36(6):1813-25. doi: 10.1093/nar/gkn015. Epub 2008 Feb 5.
5
Selenium Metabolism in Neptunia amplexicaulis. Neptunia amplexicaulis 中的硒代谢。
Plant Physiol. 1981 Feb;67(2):316-24. doi: 10.1104/pp.67.2.316.
6
Cysteinyl-tRNA Synthetase from Astragalus Species.来自黄芪属植物的半胱氨酰 - tRNA合成酶。
Plant Physiol. 1979 Jun;63(6):1095-7. doi: 10.1104/pp.63.6.1095.
7
Cysteinyl-tRNA Synthetase from Phaseolus aureus: Purification and Properties.半胱氨酰-tRNA 合成酶（Cysteinyl-tRNA Synthetase）来自 Phaseolus aureus：纯化和性质。
Plant Physiol. 1977 Nov;60(5):670-4. doi: 10.1104/pp.60.5.670.
8
Selenoprotein in Aspergillus terreus.土曲霉中的硒蛋白。
Biol Trace Elem Res. 1988 Dec;18:171-8. doi: 10.1007/BF02917501.
本文引用的文献
1
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
2
AN APPARATUS FOR PREPARATIVE TEMPERATURE-REGULATED POLYACRYLAMIDE GEL ELECTROPHORESIS.一种用于制备温度调节聚丙烯酰胺凝胶电泳的装置。
Anal Biochem. 1964 Nov;9:351-69. doi: 10.1016/0003-2697(64)90192-7.
3
An amino acid dependent exchange between 32P labeled inorganic pyrophosphate and ATP in microbial extracts.微生物提取物中32P标记的无机焦磷酸与ATP之间的氨基酸依赖性交换。
Biochim Biophys Acta. 1956 Oct;22(1):49-61. doi: 10.1016/0006-3002(56)90222-0.
4
Comparison of the chemical properties of selenocysteine and selenocystine with their sulfur analogs.硒代半胱氨酸和硒代胱氨酸与其硫类似物的化学性质比较。
Arch Biochem Biophys. 1967 Oct;122(1):164-73. doi: 10.1016/0003-9861(67)90136-1.
5
Properties of a methionyl-tRNA synthetase from Sarcina lutea.藤黄八叠球菌甲硫氨酰 - tRNA合成酶的性质
Biochim Biophys Acta. 1967 Sep 12;146(1):264-71. doi: 10.1016/0005-2744(67)90093-9.
6
The incorporation of 75-Se-selenite into dystrophogenic pasture grass. The chemical nature of the seleno compounds formed and their availability to young ovine.将75-硒亚硒酸盐掺入致营养不良的牧草中。所形成的硒化合物的化学性质及其对幼羊的有效性。
Can J Biochem. 1967 Jul;45(7):1027-39. doi: 10.1139/o67-119.
7
Biochemical differences between selenium accumulator and non-accumulator Astragalus species.富硒黄芪属植物与非富硒黄芪属植物之间的生化差异。
Biochim Biophys Acta. 1965 Aug 24;107(1):69-80. doi: 10.1016/0304-4165(65)90389-2.
8
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
9
Methionyl-tRNA synthetase detected by [75Se]selenomethionine in lenses from normal and galactose-fed rats.用[75Se]硒代蛋氨酸在正常和喂食半乳糖大鼠的晶状体中检测到甲硫氨酰-tRNA合成酶。
Exp Eye Res. 1969 Jan;8(1):84-90. doi: 10.1016/s0014-4835(69)80084-9.
10
The utilization of selenomethionine by Escherichia coli.大肠杆菌对硒代蛋氨酸的利用
Biochim Biophys Acta. 1971 Feb 23;230(2):223-36. doi: 10.1016/0304-4165(71)90207-8.
Zotero 插件