Agricultural Research Service, United States Department of Agriculture, Red River Valley Potato Research Laboratory, East Grand Forks, Minnesota 56721.
Plant Physiol. 1976 Oct;58(4):464-7. doi: 10.1104/pp.58.4.464.
Callus was initiated from explants of tubers of the Norchip cultivar of Solanum tuberosum L. and grown on medium with a single carbon source and without addition of coconut milk, protein hydrolysate, or amino acid. Callus samples were harvested at intervals and compared to mature tubers for which there was good biochemical knowledge.The amino acid spectrum, the glycoalkaloid content, and the properties of the isolated invertase and sucrose synthetase were similar in callus and in tuber. Significantly the level of sucrose synthetase varied with the age of the developing callus just as it did with the age of the developing tuber. Of greater significance, levels of reducing sugars and invertase varied with the age of developing callus and also with time and temperature of storage after the callus has ceased growth. Similar changes occur in intact tubers.Callus and tuber biochemistry differed in the amount of deposited starch and in the absence of potato invertase inhibitor.
愈伤组织是从马铃薯诺奇品种的块茎外植体诱导产生的，并在含有单一碳源且不添加椰子奶、蛋白水解物或氨基酸的培养基上生长。每隔一段时间收获愈伤组织样本，并与具有良好生化知识的成熟块茎进行比较。愈伤组织和块茎的氨基酸谱、糖苷生物碱含量以及分离的转化酶和蔗糖合酶的性质相似。重要的是，蔗糖合酶的水平随着发育愈伤组织的年龄而变化，就像发育块茎的年龄一样。更重要的是，还原糖和转化酶的水平随着发育愈伤组织的年龄以及愈伤组织停止生长后的储存时间和温度而变化。完整的块茎也会发生类似的变化。愈伤组织和块茎的生物化学在沉积淀粉的量和不存在马铃薯转化酶抑制剂方面存在差异。
