Northern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 1815 North University, Peoria, Illinois 61604.
Plant Physiol. 1976 Dec;58(6):798-9. doi: 10.1104/pp.58.6.798.
The free amino acid and ammonia pools of Azolla caroliniana were analyzed by quantitative column chromatography on columns capable of separating all of the nitrogenous constituents normally found in physiological fluids. Comparisons were made of plants containing symbiotic algae and grown on nitrogen-free media, plants grown on media containing nitrate, and algae-free plants also grown on nitrate media. The major feature of the data was a very high level of intracellular ammonia found in plants which contain N(2)-fixing algal symbionts. In addition to the more usual amino acids, serine and cystathionine were found in the free amino acid pool.
通过定量柱层析对满江红中的游离氨基酸和氨库进行分析，该柱层析可分离生理体液中通常存在的所有含氮成分。比较了共生藻类且在无氮培养基上生长的植物、在含有硝酸盐的培养基上生长的植物和在含有硝酸盐的无藻培养基上生长的植物。数据的主要特征是含有固氮藻共生体的植物细胞内氨含量非常高。除了常见的氨基酸外，游离氨基酸库中还发现了丝氨酸和半胱氨酸。
