Institute of Plant Development, University of Wisconsin, Madison, Wisconsin 53706.
Plant Physiol. 1977 Jan;59(1):45-7. doi: 10.1104/pp.59.1.45.
Cytokinin affects the requirement for auxin of a strain of tobacco callus (Nicotiana tabacum) which is cytokinin-autotrophic when grown on Murashige and Skoog medium with 11.4 mum of indole-3-acetic acid but requires cytokinin 6-(3-methyl-2-butenylamino)purine (i(6) Ade) when grown on the same medium with <3 mum indole-3-acetic acid. As the exogenous concentration of cytokinin (i(6) Ade) is increased, the concentration of indole-3-acetic acid required for growth is decreased. A second effect of cytokinin, observed sporadically in cultures with 2.5 mum or 5 mum i(6) Ade, is the transformation of some of the callus pieces to auxin-autotrophic growth. Strains, both callus-forming and bud-forming tissues, that arise in this manner are not permanently altered in their auxin requirement because subcultures on medium without cytokinin still require exogenous auxin.
细胞分裂素影响一株烟草愈伤组织（Nicotiana tabacum）对生长素的需求，当在含有 11.4 µm 吲哚-3-乙酸的 Murashige 和 Skoog 培养基上生长时，该愈伤组织是细胞分裂素自养的，但当在含有 <3 µm 吲哚-3-乙酸的相同培养基上生长时，需要细胞分裂素 6-(3-甲基-2-丁烯基氨基)嘌呤（i(6) Ade）。随着细胞分裂素（i(6) Ade）外源浓度的增加，生长所需的生长素浓度降低。细胞分裂素的第二个作用是，在含有 2.5 µm 或 5 µm i(6) Ade 的培养物中偶尔观察到的，是一些愈伤组织块向生长素自养生长的转化。以这种方式产生的愈伤组织和芽形成组织的菌株，其生长素需求并没有永久性改变，因为在没有细胞分裂素的培养基上进行继代培养仍然需要外源生长素。
