Einset J W, Skoog F
Institute of Plant Development, Birge Hall, University of Wisconsin, Madison, Wis. 53706.
Proc Natl Acad Sci U S A. 1973 Mar;70(3):658-60. doi: 10.1073/pnas.70.3.658.
A cytokinin-autotrophic strain of tobacco callus contained cytokinin-active compounds with chromatographic mobilities on Sephadex LH-20 corresponding to ribosylzeatin, zeatin, and 6-Delta(2)-isopentenylaminopurine. Zeatin, the apparent major cytokinin, was estimated to be present at a concentration of 10(-4) mumol/kg of tissue. Cytokinin-autotrophic callus supplied with [(14)C]adenine produced radioactive components with the same chromatographic properties as zeatin and 6-Delta(2)-isopentenylaminopurine. These components were not obtained from cytokinin-dependent tissue supplied with [(14)C]adenine in the same manner.
烟草愈伤组织的细胞分裂素自养型菌株含有细胞分裂素活性化合物,这些化合物在Sephadex LH - 20上的色谱迁移率与核糖基玉米素、玉米素和6 - Δ(2)-异戊烯基氨基嘌呤相对应。玉米素是明显的主要细胞分裂素,估计其在组织中的浓度为10(-4) μmol/kg。用[(14)C]腺嘌呤供应的细胞分裂素自养型愈伤组织产生了具有与玉米素和6 - Δ(2)-异戊烯基氨基嘌呤相同色谱特性的放射性成分。以同样方式用[(14)C]腺嘌呤供应的细胞分裂素依赖型组织则未获得这些成分。