Department of Botany, University of Wisconsin, 53706, Madison, Wisconsin.
Planta. 1966 Jun;72(2):146-54. doi: 10.1007/BF00387478.
High concentrations of kinetin (400-2,000 μg/l) permit continuous growth of tobacco callus cultures (Nicotiana tabacum, var. Wisconsin No. 38) in the absence of exogenous thiamine. On the optimum concentration (1,000 μg/l) the tissue has been maintained through 21 bimonthly passages without change in vigor or other growth characteristics.The effect of kinetin is general, not "mutagenic", because tissue returned to low-kinetin, thiamine-free medium failed to grow.Kinetin-thiamine interactions in "cytokinin mutant" strains which were grown without cytokinin in light and darkness suggest that the endogenous content of cytokinins may markedly affect the requirement for thiamine and possibly the tissue content of this vitamin and other growth factors.The viability of tissue on low-kinetin media in enhanced by thiamine, but the addition of this vitamin does not eliminate the requirement for a cytokinin.The great divergence in minimum kinetin concentrations required for growth of the tissue in the presence and absence of thiamine indicates that the growth promoting action of cytokinin must be different in the two cases.
高浓度的激动素（400-2000μg/l）可以在没有外源硫胺素的情况下允许烟草愈伤组织培养物（Nicotiana tabacum，var. Wisconsin No. 38）持续生长。在最佳浓度（1000μg/l）下，组织在没有活力或其他生长特性变化的情况下通过 21 次双月传代得以维持。激动素的作用是普遍的，而不是“诱变的”，因为返回低激动素、无硫胺素培养基的组织无法生长。在光和暗条件下生长而无需细胞分裂素的“细胞分裂素突变体”菌株中的细胞分裂素-硫胺素相互作用表明，细胞分裂素的内源性含量可能显著影响对硫胺素的需求以及可能影响组织中这种维生素和其他生长因子的含量。组织在低激动素培养基上的活力可以通过硫胺素增强，但添加这种维生素并不能消除对细胞分裂素的需求。在有和没有硫胺素的情况下，组织生长所需的最低激动素浓度差异很大，这表明细胞分裂素的生长促进作用在两种情况下必定不同。
