Department of Biological Sciences, Stanford University, Stanford, California 94305.
Plant Physiol. 1977 Feb;59(2):225-30. doi: 10.1104/pp.59.2.225.
Golgi dictyosomal membranes isolated from pea (Pisum sativum) stem tissue, using a combination of rate zonal and isopycnic sucrose density centrifugation, were shown to bear cytidine diphosphate-choline:diglyceride phosphorylcholinetransferase, CDP-ethanolamine:diglyceride phosphorylethanolaminetransferase, and CTP:phosphorylcholine cytidyltransferase activities. Although the majority of the activity of the phospholipid-synthesizing enzymes was associated with the endoplasmic reticulum, the activity found in the Golgi system was about 25% of the total activity. These results suggest that Golgi dictyosomes probably synthesize at least part of the membrane phospholipids that they may need for their secretory function and for dictyosomal proliferation during cell growth, rather than importing this material entirely from the endoplasmic reticulum.
使用速率区带和等密度蔗糖密度离心的组合，从豌豆（Pisum sativum）茎组织中分离出的高尔基网囊泡膜，显示出含有胞苷二磷酸胆碱：二酰基甘油磷酸胆碱转移酶、CDP-乙醇胺：二酰基甘油磷酸乙醇胺转移酶和 CTP：磷酸胆碱胞苷转移酶活性。尽管大多数磷脂合成酶的活性与内质网相关，但在高尔基系统中发现的活性约占总活性的 25%。这些结果表明，高尔基网囊泡可能合成至少一部分它们在分泌功能和细胞生长过程中进行网囊泡增殖所需的膜磷脂，而不是完全从内质网中输入这种物质。
