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1
Regulation by Lipids of Plant Microsomal Enzymes: III. Phospholipid Dependence of the Cytidine-Diphospho-Choline Phosphotransferase of Potato Microsomes.脂质对植物微粒体酶的调节：III. 马铃薯微粒体胞苷二磷酸胆碱磷酸转移酶对磷脂的依赖性
Plant Physiol. 1982 Jul;70(1):206-10. doi: 10.1104/pp.70.1.206.
2
Regulation by Lipids of Plant Microsomal Enzymes: II. LIPID DEPENDENCE OF THE NADH-CYTOCHROME c REDUCTASE OF POTATO TUBERS.植物微粒体酶的脂质调控：II. 马铃薯块茎NADH-细胞色素c还原酶的脂质依赖性
Plant Physiol. 1981 Jan;67(1):9-11. doi: 10.1104/pp.67.1.9.
3
Asymmetry of the site of choline incorporation into phosphatidylcholine of rat liver microsomes.大鼠肝微粒体中胆碱掺入磷脂酰胆碱位点的不对称性。
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4
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J Biol Chem. 1988 Feb 25;263(6):2644-50.
5
The phospholipid-dependence of uridine diphosphate glucuronyltransferase. Phospholipid depletion and re-activation of guinea-pig liver microsomal enzyme.尿苷二磷酸葡糖醛酸基转移酶的磷脂依赖性。豚鼠肝微粒体酶的磷脂耗竭与再激活。
Biochem J. 1977 Apr 1;163(1):117-24. doi: 10.1042/bj1630117.
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Phospholipid dependence of rat brain microsomal glucose-6-phosphate phosphohydrolase.大鼠脑微粒体葡萄糖-6-磷酸磷酸水解酶的磷脂依赖性
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NMDA receptor overactivation inhibits phospholipid synthesis by decreasing choline-ethanolamine phosphotransferase activity.N-甲基-D-天冬氨酸受体过度激活通过降低胆碱-乙醇胺磷酸转移酶活性来抑制磷脂合成。
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High glucose and advanced glycation end products induce phospholipid hydrolysis and phospholipid enzyme inhibition in bovine retinal pericytes.
Biochim Biophys Acta. 2001 Sep 28;1533(2):128-40. doi: 10.1016/s1388-1981(01)00151-2.

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Activation of a pea membrane protein kinase by calcium ions.钙离子激活豌豆膜蛋白激酶。
Planta. 1984 Jul;161(5):409-17. doi: 10.1007/BF00394571.
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Regulation by Phospholipids and Kinetic Studies of Plant Membrane-Bound UDP-Glucose Sterol beta-d-Glucosyl Transferase.磷脂调控及植物膜结合型 UDP-葡萄糖甾醇β-d-葡萄糖基转移酶的动力学研究。
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Proc Natl Acad Sci U S A. 1988 Jul;85(14):4966-70. doi: 10.1073/pnas.85.14.4966.
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Molecular and biochemical characterization of an aminoalcoholphosphotransferase (AAPT1) from Brassica napus: effects of low temperature and abscisic acid treatments on AAPT expression in Arabidopsis plants and effects of over-expression of BnAAPT1 in transgenic Arabidopsis.甘蓝型油菜氨基醇磷酸转移酶（AAPT1）的分子与生化特性：低温和脱落酸处理对拟南芥植株中AAPT表达的影响以及BnAAPT1在转基因拟南芥中的过表达效应
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6
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本文引用的文献

1
Identification and composition of turnip root lipids.芜菁根脂质的鉴定与组成
Lipids. 1967 May;2(3):244-50. doi: 10.1007/BF02532563.
2
Effect of polar lipids on ATPase activity of membrane preparations from germinating radish seeds.极性脂质对萝卜种子萌发时膜制剂ATP酶活性的影响。
Plant Physiol. 1981 Aug;68(2):377-81. doi: 10.1104/pp.68.2.377.
3
Phospholipid Metabolism in Plant Mitochondria: II. SUBMITOCHONDRIAL SITES OF SYNTHESIS OF PHOSPHATIDYLCHOLINE AND PHOSPHATIDYLETHANOLAMINE.植物线粒体中的磷脂代谢：II. 磷脂酰胆碱和磷脂酰乙醇胺合成的亚线粒体位点
Plant Physiol. 1981 Feb;67(2):261-5. doi: 10.1104/pp.67.2.261.
4
Regulation by Lipids of Plant Microsomal Enzymes: II. LIPID DEPENDENCE OF THE NADH-CYTOCHROME c REDUCTASE OF POTATO TUBERS.植物微粒体酶的脂质调控：II. 马铃薯块茎NADH-细胞色素c还原酶的脂质依赖性
Plant Physiol. 1981 Jan;67(1):9-11. doi: 10.1104/pp.67.1.9.
5
Phospholipid-synthesizing Enzymes Associated with Golgi Dictyosomes from Pea Tissue.豌豆组织中与高尔基体胞质小泡相关的磷脂合成酶。
Plant Physiol. 1977 Feb;59(2):225-30. doi: 10.1104/pp.59.2.225.
6
Phosphatidylcholine synthesis in castor bean endosperm.蓖麻胚乳中磷脂酰胆碱的合成。
Plant Physiol. 1976 Mar;57(3):382-6. doi: 10.1104/pp.57.3.382.
7
Studies of the electron transfer system. 47. The role of phospholipids in electron transfer.电子传递系统的研究。47. 磷脂在电子传递中的作用。
J Biol Chem. 1962 Oct;237:3264-72.
8
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
9
The function of cytidine coenzymes in the biosynthesis of phospholipides.胞苷辅酶在磷脂生物合成中的作用。
J Biol Chem. 1956 Sep;222(1):193-214.
10
[Lipid exchange between mitochondria, microsomes and cytoplasmic supernatant of potato or cauliflower cells].[马铃薯或花椰菜细胞线粒体、微粒体与细胞质上清液之间的脂质交换]
Eur J Biochem. 1970 Aug;15(2):250-62. doi: 10.1111/j.1432-1033.1970.tb01002.x.

脂质对植物微粒体酶的调节：III. 马铃薯微粒体胞苷二磷酸胆碱磷酸转移酶对磷脂的依赖性

Regulation by Lipids of Plant Microsomal Enzymes: III. Phospholipid Dependence of the Cytidine-Diphospho-Choline Phosphotransferase of Potato Microsomes.

作者信息

Jolliot A, Justin A M, Bimont E, Mazliak P

机构信息

Laboratoire de Physiologie Cellulaire, ERA 323, Université Pierre et Marie Curie, 75230 Paris cédex 05, France.

出版信息

Plant Physiol. 1982 Jul;70(1):206-10. doi: 10.1104/pp.70.1.206.

DOI:10.1104/pp.70.1.206
PMID:16662446
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1067113/
Abstract

Cytidine-diphospho-choline diacyl-glycerol phosphorylcholine phosphotransferase activity was demonstrated in potato (Solanum tuberosum L.) microsomes and the incorporation of cytidine-diphospho[(14)C]choline into phosphatidylcholine was characterized by the time course of (14)C incorporation and the effect of microsomal protein concentration on choline incorporation.Potato microsomes were progressively delipidated by treatments (2 min at 0 degrees C) with increasing amounts of phospholipase C from Bacillus cereus. A decrease in choline phosphotransferase activity was observed in parallel with the progressive hydrolysis of membrane phospholipids. A 70% (or more) phospholipid hydrolysis provoked the total inactivation of the enzyme.Adding back exogenous phospholipids (in the form of liposomes) to phospholipase C-treated membranes restored the enzymic activity. Restoration could be obtained with egg yolk phospholipids as well as with potato phospholipids. Restoration was time dependent and completed after 10 minutes; restoration was also dependent on the quantity of liposomes added to lipid-depleted membranes: the best restorations were obtained with 1 to 2.5 milligrams of phospholipid per mg of microsomal protein; higher phospholipid to protein ratios were less efficient or inhibitory.These results clearly demonstrate the phospholipid dependence of the cytidine-diphospho-choline phosphotransferase from potato microsomes.

摘要

在马铃薯（Solanum tuberosum L.）微粒体中证实了胞苷二磷酸胆碱二酰甘油磷酸胆碱磷酸转移酶活性，并且通过（14）C掺入的时间进程以及微粒体蛋白浓度对胆碱掺入的影响来表征胞苷二磷酸[（14）C]胆碱掺入磷脂酰胆碱的过程。用来自蜡状芽孢杆菌的越来越多的磷脂酶C在0℃处理（2分钟），使马铃薯微粒体逐渐脱脂。观察到胆碱磷酸转移酶活性的降低与膜磷脂的逐渐水解同时发生。70％（或更多）的磷脂水解导致该酶完全失活。将外源性磷脂（以脂质体的形式）添加回经磷脂酶C处理的膜中可恢复酶活性。用蛋黄磷脂以及马铃薯磷脂都可以实现恢复。恢复是时间依赖性的，10分钟后完成；恢复还取决于添加到脂质耗尽膜中的脂质体数量：每毫克微粒体蛋白加入1至2.5毫克磷脂可获得最佳恢复效果；更高的磷脂与蛋白比例效率较低或具有抑制作用。这些结果清楚地证明了马铃薯微粒体中胞苷二磷酸胆碱磷酸转移酶对磷脂的依赖性。