Hobbs M C, Delarge M H, Baydoun E A, Brett C T
Department of Botany, University of Glasgow, Scotland, U.K.
Biochem J. 1991 Aug 1;277 ( Pt 3)(Pt 3):653-8. doi: 10.1042/bj2770653.
The subcellular location of a glucuronyltransferase (GT) involved in glucuronoxylan synthesis in pea (Pisum sativum) has been investigated. Most of the GT activity was found in the Golgi fraction, but activity was also detected in the plasma-membrane fraction. Separation of Golgi membranes on a shallow continuous sucrose density gradient resulted in three distinct subfractions, with GT activity being confined to Golgi membranes of a density similar to that of smooth endoplasmic reticulum. The differential distribution of GT within the Golgi stack indicates that glucuronoxylan synthesis occurs in specific cisternae and that there is functional compartmentalization of the Golgi with respect to hemicellulose biosynthesis.
对豌豆（Pisum sativum）中参与葡糖醛酸木聚糖合成的一种葡糖醛酸基转移酶（GT）的亚细胞定位进行了研究。大部分GT活性存在于高尔基体部分，但在质膜部分也检测到了活性。在浅的连续蔗糖密度梯度上分离高尔基体膜产生了三个不同的亚组分，GT活性局限于密度与光滑内质网相似的高尔基体膜。GT在高尔基体堆叠中的差异分布表明，葡糖醛酸木聚糖合成发生在特定的潴泡中，并且高尔基体在半纤维素生物合成方面存在功能区室化。
