Department of Botany, University of California, Davis, California 95616.
Plant Physiol. 1977 Mar;59(3):376-80. doi: 10.1104/pp.59.3.376.
The role of phloem turgor pressure in (14)C-assimilate translocation in Ecballium elaterium A. Rich was studied. The direction of translocation was manipulated by two methods: darkening, or defoliation, of the upper or lower halves of the shoots. After 24 hours of labeled assimilate movement, sieve tube turgor levels were measured with the phloem needle technique. Distribution of label, determined by autoradiography and counting, revealed a direct correlation between the direction of assimilate transport and the pressure difference. Phloem turgor levels always decreased in the stem of darkened shoots; this resulted in greater pressure differences in the stem between the source leaf receiving (14)CO(2) and treated regions.
研究了薛荔(Ecballium elaterium A. Rich)韧皮部膨压在(14)C 同化物运转中的作用。通过两种方法来操纵同化物运转的方向:将茎的上半部分或下半部分遮暗或摘叶。在标记同化物运转 24 小时后,用韧皮部针技术测量筛管膨压水平。通过放射自显影和计数确定的标记分布显示,同化物运输的方向与压力差之间存在直接的相关性。在遮暗的茎中,韧皮部膨压水平总是下降;这导致在源叶接受(14)CO2 和处理区域之间的茎中产生更大的压力差。