Walker-Simmons M, Ryan C A
Department of Agricultural Chemistry and Program in Biochemistry and Biophysics, Washington State University, Pullman, Washington 99163.
Plant Physiol. 1977 Mar;59(3):437-9. doi: 10.1104/pp.59.3.437.
Proteinase inhibitor-inducing factor (PIIF)-induced accumulation of trypsin inhibitory activity was assayed in leaves of 23 species of plants representing 10 agriculturally important genera. Inhibitory activity was assayed in extracts from attached leaves or from excised leaves supplied through the cut petioles for 30 minutes with extracts containing the wound hormone PIIF, obtained from either tomato leaves or from the leaves of each plant under study. During subsequent incubation in light for 72 hours, PIIF-induced trypsin inhibitory activity accumulated in significant quantities in 10 of the 23 species. Alfalfa accumulated the highest levels of inhibitory activity (340 mug trypsin inhibited/ml leaf juice), followed by tobacco, tomato, potato, strawberry, cucumber, squash, clover, broadbean, and grape. It is suggested that the inhibitors might be classed as allelochemics that are present in certain plants and not others in response to environmental pressures during their evolution.
在代表10个重要农业属的23种植物的叶片中,检测了蛋白酶抑制剂诱导因子(PIIF)诱导的胰蛋白酶抑制活性积累情况。抑制活性在附着叶片的提取物中进行检测,或者在通过叶柄切口用含有创伤激素PIIF的提取物供应30分钟的离体叶片中进行检测,PIIF提取物取自番茄叶片或所研究的每种植物的叶片。在随后72小时的光照培养过程中,PIIF诱导的胰蛋白酶抑制活性在23种植物中的10种中大量积累。苜蓿积累的抑制活性水平最高(每毫升叶汁抑制340微克胰蛋白酶),其次是烟草、番茄、马铃薯、草莓、黄瓜、南瓜、三叶草、蚕豆和葡萄。有人认为,这些抑制剂可能被归类为化感物质,它们存在于某些植物中,而不存在于其他植物中,这是植物在进化过程中对环境压力的一种反应。