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离体诱导的马铃薯小叶中胰凝乳蛋白酶抑制剂I蛋白的合成。

Synthesis of chymotrypsin inhibitor I protein in potato leaflets induced by detachment.

作者信息

Ryan C A

出版信息

Plant Physiol. 1968 Nov;43(11):1859-65. doi: 10.1104/pp.43.11.1859.

DOI:10.1104/pp.43.11.1859
PMID:5699150
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1087090/
Abstract

Chymotrypsin inhibitor I is a protein that can be induced to accumulate in potato leaflets within hr in the light when leaflets are detached from intact plants and supplied with water. This increase in inhibitor I is not accompanied by an increase in all proteins in the detached leaflets. The accumulation is polar, proceeding from the base of the leaflets toward the tip. Low inhibitor I levels can be effectively maintained in detached leaflets by supplying them either water in the dark or solutions of indole acetic acid or 2,4-dichlorophenoxy acetic acid in the light. Inhibitor I from leaflets is not identical to inhibitor I isolated from potato tubers as determined by immunochemical analyses.

摘要

胰凝乳蛋白酶抑制剂I是一种蛋白质，当将马铃薯小叶从完整植株上摘下并供水时，在光照下数小时内可诱导其在小叶中积累。抑制剂I的这种增加并未伴随离体小叶中所有蛋白质的增加。积累是极性的，从小叶基部向尖端进行。通过在黑暗中给离体小叶供水或在光照下给其提供吲哚乙酸或2,4-二氯苯氧基乙酸溶液，可以有效地维持其低抑制剂I水平。通过免疫化学分析确定，小叶中的抑制剂I与从马铃薯块茎中分离出的抑制剂I不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1c3/1087090/b78e658967e5/plntphys00490-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1c3/1087090/d72d5d922968/plntphys00490-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1c3/1087090/b78e658967e5/plntphys00490-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1c3/1087090/d72d5d922968/plntphys00490-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1c3/1087090/b78e658967e5/plntphys00490-0117-a.jpg

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本文引用的文献

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