Knipple D C, Payne L L, Soderlund D M
Department of Entomology, New York State Agricultural Experiment Station, Cornell University, Geneva 14456.
Arch Insect Biochem Physiol. 1991;16(1):45-53. doi: 10.1002/arch.940160106.
A segment of the house fly (Musca domestica) homologue of the para (paralytic) sodium channel gene of Drosophila melanogaster was isolated by using mixed sequence oligonucleotide primers in the polymerase chain reaction (PCR). The specificity of the procedure was demonstrated by genomic Southern analysis using the housefly PCR amplification product as a probe and by DNA sequence analysis. The latter showed structural homology to the para gene, but not to the corresponding region of DSC1, another D. melanogaster gene with structural similarity to vertebrate sodium channel genes.
利用混合序列寡核苷酸引物通过聚合酶链反应（PCR）分离出了家蝇（Musca domestica）中与黑腹果蝇（Drosophila melanogaster）的para（麻痹）钠通道基因同源的一段序列。通过使用家蝇PCR扩增产物作为探针进行基因组Southern分析以及DNA序列分析，证明了该方法的特异性。DNA序列分析表明，该序列与para基因具有结构同源性，但与DSC1（黑腹果蝇的另一个与脊椎动物钠通道基因结构相似的基因）的相应区域无结构同源性。
