利用高效液相色谱法从植物组织中快速分离和定量脱落酸。
Rapid separation and quantification of abscisic Acid from plant tissues using high performance liquid chromatography.
机构信息
University of Minnesota, St. Paul, Minnesota 55108.
出版信息
Plant Physiol. 1977 May;59(5):821-6. doi: 10.1104/pp.59.5.821.
Abstract
Abscisic acid (ABA) was purified from soybean (Glycine max [L.]) seed extract using a preparative high performance liquid chromatography (HPLC) procedure. The preparative procedure was rapid (70 minutes per sample), required no prior partitioning for purification and was quantitative as demonstrated with an internal standard of [2-(14)C]ABA, of which 98.9% was recovered.Following purification by the preparative HPLC procedure, the ABA in a soybean seed extract was quantified using either GLC with an electron capture detector (GLC-EC) or by analytical HPLC with a UV detector. For soybean seed extracts, two analytical HPLC column packing materials were found adequate: muPorasil and muBondapak-NH(2) (Waters Associates). However, with complex tissue extracts, such as soybean leaf and nodule tissues, only GLC-EC had the necessary selectivity and sensitivity.
摘要
用制备高效液相色谱（HPLC）程序从大豆（Glycine max [L.])种子提取物中纯化脱落酸（ABA）。该制备程序快速（每个样品 70 分钟），不需要预先分配即可进行纯化，并且用[2-（14）C]ABA 的内标进行定量，其中 98.9%被回收。通过制备 HPLC 程序纯化后，使用带有电子俘获检测器的 GLC（GLC-EC）或带有紫外检测器的分析 HPLC 定量大豆种子提取物中的 ABA。对于大豆种子提取物，发现两种分析 HPLC 柱填料材料足够：muPorasil 和 muBondapak-NH(2)（Waters Associates）。然而，对于复杂的组织提取物，如大豆叶片和根瘤组织，只有 GLC-EC 具有必要的选择性和灵敏度。
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