Liang Hao, Li Chunfang, Yuan Qipeng, Vriesekoop Frank
Key Laboratory of Bioprocess of Beijing, Beijing University of Chemical Technology, Beijing 100029, PR China.
J Agric Food Chem. 2007 Oct 3;55(20):8047-53. doi: 10.1021/jf0706833. Epub 2007 Sep 7.
A novel, rapid, and economical method to isolate and purify natural sulforaphane from broccoli seeds is described. The procedure involves solvent extraction of autolyzed seed meal, followed by separation by solid phase extraction (SPE) and purification by preparative high-performance liquid chromatography (HPLC). The SPE method provides higher yield of sulforaphane from crude extracts compared to conventional liquid-liquid extraction. High purity and recovery of sulforaphane product can be obtained by preparative HPLC with a C 18 column and 30% methanol in water as the mobile phase. The purified compound was characterized by MS and (1)H and (13)C NMR. The techniques described here are useful tools in the preparative-scale isolation of sulforaphane in a fast, cost-effective, and waste-conscious manner.
本文描述了一种从西兰花种子中分离和纯化天然萝卜硫素的新颖、快速且经济的方法。该方法包括对自溶的种子粉进行溶剂萃取，然后通过固相萃取（SPE）进行分离，并通过制备型高效液相色谱（HPLC）进行纯化。与传统的液 - 液萃取相比，SPE方法能从粗提物中获得更高产量的萝卜硫素。使用C18柱和30%甲醇水溶液作为流动相的制备型HPLC可获得高纯度和高回收率的萝卜硫素产品。纯化后的化合物通过质谱（MS）以及氢谱（1H）和碳谱（13C）核磁共振进行表征。这里描述的技术是以快速、经济高效且注重环保的方式在制备规模上分离萝卜硫素的有用工具。
