Departments of Biology and Biochemistry, University of California, Riverside, California 92502.
Plant Physiol. 1977 May;59(5):911-4. doi: 10.1104/pp.59.5.911.
It was found that in the alga, Chlorella sorokiniana, mannitol penetrated the plasmalemma (normally thought to be impermeable to mannitol) into the intracellular space. The rate of penetration is exponential and relatively slow, having a half-time of 6 to 12 minutes and requiring over 60 minutes for complete penetration. This penetration was demonstrated both by the Millipore filtration of cells incubated with (14)C-mannitol and by centrifugation of the cells through a silicon oil layer after incubation with tritiated water and (14)C-mannitol. Further, mannitol caused an inhibition of both autotrophic (on CO(2)) and heterotrophic (on glucose) growth. A low rate of mannitol metabolism was demonstrated, although this rate could not support heterotrophic growth.
研究发现，在小球藻中，甘露醇穿透质膜（通常被认为对甘露醇不透）进入细胞内空间。穿透速度呈指数增长且相对较慢，半衰期为 6 到 12 分钟，完全穿透需要 60 多分钟。这一渗透过程通过用 (14)C-甘露醇孵育细胞的 Millipore 过滤和用氚化水和 (14)C-甘露醇孵育后通过硅油层离心细胞得到了证实。此外，甘露醇抑制了自养（在 CO(2)上）和异养（在葡萄糖上）的生长。尽管该速率不能支持异养生长，但证明了甘露醇的代谢率较低。
