Charria-Girón Esteban, Amazo Vanessa, De Angulo Daniela, Hidalgo Eliana, Villegas-Torres María Francisca, Baganz Frank, Caicedo Ortega Nelson H
Departamento de Ingeniería Bioquímica, Facultad de Ingeniería, Universidad Icesi, Calle 18 No. 122-135, Cali 760031, Colombia.
Departamento de Ciencias Químicas, Facultad de Ciencias Naturales, Universidad Icesi, Calle 18 No. 122-135, Cali 760031, Colombia.
Bioengineering (Basel). 2021 Jun 10;8(6):82. doi: 10.3390/bioengineering8060082.
Microalgae provides an alternative for the valorization of industrial by-products, in which the nutritional content varies substantially and directly affects microalgae system performance. Herein, the heterotrophic cultivation of was systematically studied, allowing us to detect a nutritional deficiency other than the carbon source through assessing the oxygen transfer rate for glucose or acetate fermentation. Consequently, a mathematical model of the iron co-limiting effect on heterotrophic microalgae was developed by exploring its ability to regulate the specific growth rate and yield. For instance, higher values of the specific growth rate (0.17 h) compared with those reported for the heterotrophic culture of were obtained due to iron supplementation. Therefore, anaerobic sludge from an industrial wastewater treatment plant (a baker's yeast company) was pretreated to obtain an extract as a media supplement for . According to the proposed model, the sludge extract allowed us to supplement iron values close to the growth activation concentration (K ~12 mg L). Therefore, a fed-batch strategy was evaluated on nitrogen-deprived cultures supplemented with the sludge extract to promote biomass formation and fatty acid synthesis. Our findings reveal that nitrogen and iron in sludge extract can supplement heterotrophic cultures of and provide an alternative for the valorization of industrial anaerobic sludge.
微藻为工业副产品的增值利用提供了一种替代方案，其中营养成分差异很大，直接影响微藻系统性能。在此，对[具体微藻名称未给出]的异养培养进行了系统研究，通过评估葡萄糖或醋酸盐发酵的氧传递速率，使我们能够检测除碳源之外的营养缺乏情况。因此，通过探索其调节比生长速率和产量的能力，建立了铁对异养微藻共限制作用的数学模型。例如，由于添加了铁，获得了比报道的[具体微藻名称未给出]异养培养更高的比生长速率值（0.17 h⁻¹）。因此，对来自一家工业废水处理厂（一家面包酵母公司）的厌氧污泥进行预处理，以获得一种提取物作为[具体微藻名称未给出]的培养基补充剂。根据所提出的模型，污泥提取物使我们能够补充接近生长激活浓度（Kₛ ~12 mg L⁻¹）的铁值。因此，对添加了污泥提取物的缺氮培养物评估了补料分批策略，以促进生物质形成和脂肪酸合成。我们的研究结果表明，污泥提取物中的氮和铁可以补充[具体微藻名称未给出]的异养培养，并为工业厌氧污泥的增值利用提供了一种替代方案。
