小球藻葡萄糖转运的组成型酶系统
A Constitutive Enzyme System for Glucose Transport by Chlorella sorokiniana.
作者信息
Heath R L
机构信息
Department of Botany and Plant Sciences, University of California, Riverside, California 92521.
出版信息
Plant Physiol. 1979 Aug;64(2):224-7. doi: 10.1104/pp.64.2.224.
It was found that the transport system for glucose (as measured by deoxyglucose uptake) in the high temperature strain of Chlorella (strain 07-11-05 or C. sorokiniana) was constitutive and the rate of uptake did not increase upon incubation of autotrophically grown cells with either deoxyglucose or glucose. The uptake obeyed Michaelis-Menten type kinetics with a concentration of 200 micromolar for half-saturation. The maximum rate of uptake was nearly 10 times faster per cell (at 38 C) than that reported for any other Chlorella. This rapid accumulation of deoxyglucose causes the passive efflux to become significant compared to the pump-driven influx and nonlinear uptake appears even after only 3 to 4 minutes.
研究发现，小球藻高温菌株（07-11-05菌株或索氏小球藻）中的葡萄糖转运系统（通过脱氧葡萄糖摄取来衡量）是组成型的，自养生长的细胞在与脱氧葡萄糖或葡萄糖一起孵育时，摄取速率不会增加。摄取遵循米氏动力学类型，半饱和浓度为200微摩尔。每个细胞的最大摄取速率（在38℃时）比报道的任何其他小球藻快近10倍。这种脱氧葡萄糖的快速积累导致被动流出与泵驱动的流入相比变得显著，甚至仅在3至4分钟后就出现非线性摄取。
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