Department of Biological Sciences, Northwestern University, Evanston, Illinois 60201.
Plant Physiol. 1977 Nov;60(5):765-6. doi: 10.1104/pp.60.5.765.
Gametophore induction in moss by Agrobacterium tumefaciens was inhibited by addition of lipopolysaccharide (LPS) from A. tumefaciens. The LPS did not affect bacterial viability or appear to bind to bacterial cells. LPS from nonbinding Agrobacterium radiobacter was not effective in reducing gametophore formation. A. tumefaciens LPS, if added 24 hours after addition of viable bacterial cells, had no effect in reducing gametophore formation. The polysaccharide portion of the LPS was identified as the binding component necessary for attachment of agrobacteria for induction of gametophores in moss and tumors in higher plants.
农杆菌介导的藓配子体诱导被脂多糖(LPS)抑制,该 LPS 来自于农杆菌。LPS 并不影响细菌的活力,也似乎不与细菌细胞结合。非结合型根癌农杆菌的 LPS 并不能有效减少配子体的形成。如果在加入有活力的细菌细胞 24 小时后加入 LPS,则对减少配子体的形成没有影响。LPS 的多糖部分被鉴定为附着成分,对于附着农杆菌以诱导藓配子体和高等植物肿瘤是必需的。