Improved genome of type strain provides new taxonomic insight into genomospecies 4.

The reported DSM 30174 genome is highly fragmented, hindering robust comparative genomics and genome-based taxonomic analysis. We re-sequenced the type strain, generating a dramatically improved genome with high contiguity. In addition, we sequenced the genome of B6, enabling for the first time, a proper comparative genomics of these contentious species. We provide concrete evidence that the previously reported type strain genome (Accession Number: ASXY01) is contaminated which explains its abnormally large genome size and fragmented assembly. We propose that be reclassified as subsp. and that retains it species status with the proposed name of subsp. . This proposal is based, first on the high pairwise genome-scale average nucleotide identity supporting the amalgamation of both and into a single species. Second, maximum likelihood tree construction based on the concatenated alignment of shared genes (core genes) among related strains indicates that NCPPB3001 is sufficiently divergent from to propose two independent sub-clades. Third, demonstrates the genomic potential to synthesize the L configuration of fucose in its lipid polysaccharide, fostering its ability to colonize plant cells more effectively than