Soliday C L, Dean B B, Kolattukudy P E
Department of Agricultural Chemistry and the Program in Biochemistry and Biophysics, Washington State University, Pullman, Washington 99164.
Plant Physiol. 1978 Feb;61(2):170-4. doi: 10.1104/pp.61.2.170.
Wounding of potato (Solanum tuberosum L.) tubers results in suberization, apparently triggered by the release of some chemical factor(s) at the cut surface. Suberization, as measured by diffusion resistance of the tissue surface to water vapor, was inhibited by mm concentrations of indoleacetic acid, unaffected by mm concentrations of traumatic acid, severely inhibited at mum concentrations of cytokinin, but stimulated by abscisic acid (ABA) at 10(-4)m. Thorough washing of potato disks up to 3 to 4 days after cutting resulted in severe inhibition of suberization as measured both by diffusion resistance and by the amount of the octadecene diol generated by hydrogenolysis (LiAlH(4)) of the tissue. Disks washed after 4 days did not show any inhibition of suberization. High performance liquid chromatographic analysis of the wash from fresh potato disks showed that about 14 ng of ABA was released into the wash per g of tissue. The amount of ABA released increased with time up to 4 to 6 hours of washing. The maximal amount of ABA was washed out after aging for 24 hours and after 2 days of aging ABA could no longer be found in the surface wash of the disks. Addition of ABA to the media of potato tissue cultures resulted in suberin formation whereas control cultures contained little suberin. The effect of ABA on suberization in the tissue cultures was shown to be linearly concentration-dependent up to 10(-4)m and a linear increase in suberin formation was seen up to about 8 days of culture growth on the media containing 10(-4)m ABA. From these results it is proposed that during the early phase of wound-healing ABA plays a role in triggering a chain of biochemical processes which eventually (in about 3 to 4 days) result in the formation of a suberization-inducing factor, responsible for the induction of the enzymes involved in suberin biosynthesis.
马铃薯（Solanum tuberosum L.）块茎受伤会导致木栓化，这显然是由切割表面释放的某些化学因子触发的。通过组织表面对水蒸气的扩散阻力来衡量，毫米浓度的吲哚乙酸会抑制木栓化，毫米浓度的创伤酸对其没有影响，微克浓度的细胞分裂素会严重抑制木栓化，但10⁻⁴摩尔的脱落酸（ABA）会刺激木栓化。切割后3至4天对马铃薯切片进行彻底冲洗，无论是通过扩散阻力还是通过对组织进行氢解（LiAlH₄）产生的十八碳二醇的量来衡量，都会导致木栓化受到严重抑制。4天后冲洗的切片未表现出对木栓化的任何抑制作用。对新鲜马铃薯切片冲洗液进行高效液相色谱分析表明，每克组织约有14纳克的ABA释放到冲洗液中。ABA的释放量随着冲洗时间的延长而增加，直至冲洗4至6小时。老化24小时后ABA被冲洗出的量最大，老化2天后在切片的表面冲洗液中再也找不到ABA。向马铃薯组织培养物的培养基中添加ABA会导致木栓质形成，而对照培养物中几乎没有木栓质。ABA对组织培养物中木栓化的影响在高达10⁻⁴摩尔时呈线性浓度依赖性，在含有10⁻⁴摩尔ABA的培养基上培养约8天时，木栓质形成呈线性增加。从这些结果可以推测，在伤口愈合的早期阶段，ABA在触发一系列生化过程中起作用，这些过程最终（约3至4天）导致形成一种木栓化诱导因子，该因子负责诱导参与木栓质生物合成的酶。
