荷荷巴幼苗子叶中蜡酯水解酶的发育与特性
Development and properties of a wax ester hydrolase in the cotyledons of jojoba seedlings.
作者信息
Huang A H, Moreau R A, Liu K D
机构信息
Department of Biology, University of South Carolina, Columbia, South Carolina 29208.
出版信息
Plant Physiol. 1978 Mar;61(3):339-41. doi: 10.1104/pp.61.3.339.
The activity of a wax ester hydrolase in the cotyledons of jojoba (Simmondsia chinensis) seedlings increased drastically during germination, parallel to the development of the gluconeogenic process. The enzyme at its peak of development was obtained in association with the wax body membrane, and its properties were studied. It had an optimal activity at alkaline pH (8.5-9). The apparent K(m) value for N-methylindoxylmyristate was 93 muM. It was stable at 40 C for 30 min but was inactivated at higher temperature. Various divalent cations and ethylenediaminetetraacetate had little effect on the activity. p-Chloromercuribenzoate was a strong inhibitor of the enzyme activity, and its effect was reversed by subsequent addition of dithiothreitol. It had a broad substrate specificity with highest activities on monoglycerides, wax esters, and the native substrate (jojoba wax).
荷荷巴（Simmondsia chinensis）种子幼苗子叶中蜡酯水解酶的活性在萌发过程中急剧增加，这与糖异生过程的发展平行。在蜡质体膜相关部位获得了处于发育高峰期的该酶，并对其性质进行了研究。它在碱性pH（8.5 - 9）下具有最佳活性。N - 甲基吲哚基肉豆蔻酸酯的表观K(m)值为93 μM。它在40℃下30分钟内稳定，但在更高温度下失活。各种二价阳离子和乙二胺四乙酸对活性影响很小。对氯汞苯甲酸是该酶活性的强抑制剂，随后添加二硫苏糖醇可逆转其作用。它具有广泛的底物特异性，对甘油单酯、蜡酯和天然底物（荷荷巴蜡）具有最高活性。
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