花生碱性脂肪酶
Peanut alkaline lipase.
作者信息
Sanders T H, Pattee H E
出版信息
Lipids. 1975 Jan;10(1):50-4. doi: 10.1007/BF02532194.
PMID:235692
Abstract
Peanut alkaline lipase, (glycerol ester hydrolase EC 3.1.1.3), pH optimum 8.5, was isolated from acetone powders prepared from developing and germinated peanut seed (arachis hypogaea L. var. NC-2). Enzyme activity/seed increased in successive developmental stages. The course of the hydrolytic reaction was linear with regard to enzyme concentration and all times tested up to periods exceeding 60 min. Km for the reaction was determined to be 2.6 times 10-4M. Molecular weight of peanut lipase, as estimated by Sephadex gel filtration and sodium dodecyl sulfate gel electrophoresis, was ca. 55,000.
摘要
花生碱性脂肪酶（甘油酯水解酶，EC 3.1.1.3），最适pH值为8.5，是从发育中和发芽的花生种子（落花生L.变种NC-2）制备的丙酮粉中分离出来的。酶活性/种子在连续发育阶段增加。水解反应过程在酶浓度和所有测试时间（长达超过60分钟）方面呈线性。该反应的米氏常数经测定为2.6×10⁻⁴M。通过葡聚糖凝胶过滤和十二烷基硫酸钠凝胶电泳估计，花生脂肪酶的分子量约为55,000。
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