在整体细胞和原生质体中的 RNA 合成：比较。
RNA synthesis in whole cells and protoplasts of centaurea: a comparison.
机构信息
Department of Biological Sciences, State University of New York at Albany, Albany, New York 12222.
出版信息
Plant Physiol. 1978 Apr;61(4):575-80. doi: 10.1104/pp.61.4.575.
Protoplasts enzymically isolated from suspension cultures of Centaurea cyanus L. incorporate radioactive precursors into RNA with kinetics similar to that of whole cells. There are differences, however, in several other aspects of RNA metabolism. The proportion of total RNA that contains poly(A) sequences (25 to 30%) is similar in both freshly isolated protoplasts and whole cells after a 20-minute pulse with [(3)H]adenosine. After a 4-hour pulse, however, poly(A)-containing RNA makes up 30% of the total RNA in protoplasts whereas it drops to 8% in whole cells. There appears to be a faulty processing of ribosomal precursor into the mature ribosomal species, as the precursor seems to accumulate to higher levels relative to the mature 18S and 25S rRNAs in protoplasts as compared to whole cells. Additional differences are seen in the size distributions of poly(A)-containing RNA, although the length of the poly(A) segment is similar in both protoplasts and whole cells. Within 24 hours protoplasts appear to have resumed a pattern of RNA synthesis similar to that of whole cells.
从矢车菊悬浮培养物中酶解分离的原生质体以类似于完整细胞的动力学将放射性前体掺入 RNA 中。然而，在 RNA 代谢的几个其他方面存在差异。用 [(3)H]腺苷进行 20 分钟脉冲后，新鲜分离的原生质体和完整细胞中总 RNA 中含有 poly(A) 序列的比例（25%至 30%）相似。然而，4 小时脉冲后，含 poly(A)的 RNA 占原生质体总 RNA 的 30%，而在完整细胞中则降至 8%。似乎存在核糖体前体向成熟核糖体物种的加工错误，因为与完整细胞相比，前体似乎在原生质体中积累到更高水平，相对于成熟的 18S 和 25S rRNA。在含 poly(A)的 RNA 的大小分布中也观察到其他差异，尽管 poly(A) 片段的长度在原生质体和完整细胞中相似。在 24 小时内，原生质体似乎恢复了类似于完整细胞的 RNA 合成模式。
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