Laboratoire de Virologie, Institut de Biologie Moléculaire et Cellulaire, C.N.R.S., 15, Rue Descartes, F-67000, Strasbourg, France.
Planta. 1979 Jan;145(3):279-85. doi: 10.1007/BF00454453.
The biosynthesis of Fraction I protein in isolated protoplasts is compared with that in the plant. Radioactive precursors were incorporated into isolated protoplasts ("in vitro" labeling) and into leaves, from which the protoplasts were isolated later ("in situ" labeling). The biosynthesis of Fraction I protein stopped almost completely as soon as the protoplasts were incubated in the culture medium.
分离原生质体中 I 型蛋白的生物合成与植物中的生物合成进行了比较。放射性前体被掺入分离的原生质体(“体外”标记)和后来从中分离原生质体的叶片(“原位”标记)中。一旦原生质体在培养基中孵育,I 型蛋白的生物合成几乎完全停止。