Botany School, South Parks Road, Oxford OX1 3RA, United Kingdom.
Plant Physiol. 1978 Jun;61(6):957-60. doi: 10.1104/pp.61.6.957.
The catalase activity of unwashed preparations containing intact spinach (Spinacia oleracea L.) chloroplasts is inhibited both by cyanide and by azide at concentrations which also cause inhibition of photosynthetic CO(2)- dependent O(2) evolution.Aminotriazole can also be used to inhibit this contaminant catalase, and in this case inhibition of catalase can be achieved at aminotriazole concentrations which have little effect on the rate of photosynthetic CO(2) fixation. Aminotriazole may be used as a specific inhibitor of catalase in order to demonstrate inhibition of photosynthesis by added H(2)O(2).It is therefore concluded that inhibition of photosynthesis by cyanide and azide does not necessarily result from inhibition of catalase in the chloroplast preparation, and that intact chloroplasts do not produce inhibitory concentrations of H(2)O(2) under the best experimental conditions for CO(2) fixation.
未洗涤的含有完整菠菜（Spinacia oleracea L.）叶绿体的制剂中的过氧化氢酶活性被氰化物和叠氮化物抑制，抑制浓度也会导致光合 CO2 依赖性 O2 释放的抑制。氨基三唑也可用于抑制这种污染的过氧化氢酶，在这种情况下，氨基三唑的浓度可以抑制过氧化氢酶，而对光合 CO2 固定的速率影响很小。氨基三唑可用作过氧化氢酶的特异性抑制剂，以证明添加 H2O2 对光合作用的抑制作用。因此，结论是，氰化物和叠氮化物对光合作用的抑制不一定是由于叶绿体制剂中过氧化氢酶的抑制，而且在 CO2 固定的最佳实验条件下，完整的叶绿体不会产生抑制浓度的 H2O2。
