多胺调控燕麦叶片原生质体核糖核酸酶活性的双重机制。
Dual Mechanisms in Polyamine-mediated Control of Ribonuclease Activity in Oat Leaf Protoplasts.
机构信息
Department of Biology, Yale University, New Haven, Connecticut 06520.
出版信息
Plant Physiol. 1978 Jul;62(1):158-60. doi: 10.1104/pp.62.1.158.
Abstract
Dibasic amino acids and polyamines added to oat (Avena sativa L.) leaf protoplast isolation media decrease the RNase activity of extracted protoplasts relative to controls. This effect, which is manifested even when the added polyamine is removed by exhaustive dialysis prior to assay, is due to a prevention of the rise in RNase activity which usually follows protoplast isolation. Polyamines, but not dibasic amino acids, also decrease RNase activity in vitro. This in vitro effect seems to result from electrovalent attachment of the polyamine to the RNA, because the greater the net positive charge on the polyamine, the greater is its inhibitory effect in vitro. The activity of dibasic amino acids when added during protoplast isolation probably results from their conversion to polyamines.
摘要
添加到燕麦（Avena sativa L.）叶原生质体分离培养基中的二碱基氨基酸和多胺可降低与对照相比提取原生质体的核糖核酸酶活性。这种效应即使在测定前通过反复透析去除添加的多胺也会显现出来，这是由于防止了通常在原生质体分离后核糖核酸酶活性的升高。多胺，但不是二碱基氨基酸，也可降低体外的核糖核酸酶活性。这种体外效应似乎是由于多胺与 RNA 之间的电价附着所致，因为多胺的净正电荷越大，其体外抑制作用就越大。添加到原生质体分离过程中的二碱基氨基酸的活性可能是由于它们转化为多胺的结果。
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本文引用的文献
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Stabilization of Oat Leaf Protoplasts through Polyamine-mediated Inhibition of Senescence.通过多胺介导的衰老抑制稳定燕麦叶原生质体。
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2
Studies on ribonuclease in tobacco leaves. I. Purification and properties.烟草叶片中核糖核酸酶的研究。I. 纯化及性质
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A note on the effect of spermine on degradation of pyrimidine polynucleotides by pancreatic ribonuclease.关于精胺对胰腺核糖核酸酶降解嘧啶多核苷酸作用的注释
Acta Biochim Pol. 1967;14(1):163-8.
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