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二胺和多胺对植物悬浮培养物衰老的影响。

Influence of diamines and polyamines on the senescence of plant suspension cultures.

机构信息

Department of Botany and Microbiology, University of Oklahoma, 73019, Norman, OK, USA.

出版信息

Plant Cell Rep. 1983 Apr;2(2):82-4. doi: 10.1007/BF00270171.

Abstract

The diamines putrescine and cadaverine and the polyamines spermine and spermidine inhibited the senescence of nonphotosynthetic cultures of Paul's Scarlet rose. Response was observed when the media of stationary phase cultures was adjusted to either 1 mM of cadaverine or putrescine; or 0.1 μM of either spermine or spermidine along with 2% sucrose in all cases. Senescence of the cultures was followed by microscopic examination of cell aliquots removed at 10 day intervals and treated with the vital stain, fluorescein diacetate.

摘要

腐胺、尸胺、亚精胺和精胺抑制非光合培养的保尔氏蔷薇的衰老。当将静止期培养物的培养基调节为 1mM 尸胺或腐胺;或在所有情况下 0.1μM 的亚精胺或精胺,以及 2%蔗糖时,观察到了反应。通过每隔 10 天取出细胞等分试样并用荧光素二乙酸酯进行活细胞染色,来跟踪培养物的衰老。

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