亚硫酸盐对罂粟叶肉细胞新陈代谢的影响
Effects of Sulfite on Metabolism in Isolated Mesophyll Cells from Papaver somniferum.
作者信息
Paul J S, Bassham J A
机构信息
Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, University of California, Berkeley, California 94720.
出版信息
Plant Physiol. 1978 Aug;62(2):210-4. doi: 10.1104/pp.62.2.210.
Exposure (30 minutes) of leaf-free mesophyll cells from the C-3 plant, Papaver somniferum, to concentrations of sulfite (SO(2) + HSO(3) (-) + SO(3) (-)) up to 20 millimolar stimulated the rate of CO(2) incorporation as much as 30%. The sulfite rapidly affects the metabolism of newly incorporated CO(2). Ammonia incorporation into glutamine and subsequent transamination reactions were stimulated during the short term exposure periods while glycolate metabolism apparently was inhibited by bisulfite at two points in the pathway. The results further indicate that glycolate is the major precursor of glycine in these cells. Prolonged periods of exposure (24 hours) to sulfite had somewhat different effects on carbon metabolism: the high concentrations (10 to 20 millimolar) severely inhibited all aspects of cellular metabolism while lower concentrations (1 millimolar) appeared to inhibit ammonia incorporation but stimulated synthesis of sucrose and starch.
将C-3植物罂粟无叶叶肉细胞暴露于浓度高达20毫摩尔的亚硫酸盐（SO₂ + HSO₃⁻ + SO₃²⁻）中30分钟，可使二氧化碳固定速率提高多达30%。亚硫酸盐迅速影响新固定二氧化碳的代谢。在短期暴露期间，氨掺入谷氨酰胺及随后的转氨反应受到刺激，而在途径中的两个点，亚硫酸氢盐明显抑制了乙醇酸代谢。结果还表明，乙醇酸是这些细胞中甘氨酸的主要前体。长时间（24小时）暴露于亚硫酸盐对碳代谢有不同影响：高浓度（10至20毫摩尔）严重抑制细胞代谢的各个方面，而低浓度（1毫摩尔）似乎抑制氨掺入，但刺激蔗糖和淀粉的合成。
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