Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, University of California, Berkeley, California 94720.
Plant Physiol. 1977 Nov;60(5):775-8. doi: 10.1104/pp.60.5.775.
The establishment and maintenance of high rates of photosynthetic CO(2) incorporation in mesophyll cells of Papaver somniferum (opium poppy) depend on a regime of dark and light periods immediately following isolation, as well as carefully adjusted conditions of isolation. Analysis of the incorporation pattern of (14)CO(2) by the isolated cells indicates an initial "stress-response" period of approximately 20 hours characterized by increased respiratory-type metabolism and diminished photosynthesis. Under the favorable regime, this period is followed by rapid recovery and the reinstatement of a metabolic state strikingly similar to that of intact leaves in which the initial rate of CO(2) incorporation is between 110 and 175 mumoles CO(2) fixed per mg chlorophyll per hour. The photosynthetic viability of these cells can be maintained for up to 80 hours.
在罂粟（鸦片罂粟）的叶肉细胞中建立和维持高比例的光合 CO2 同化，需要在分离后紧接着的暗期和光期的特定制度，以及精心调整的分离条件。对分离细胞中（14）CO2 掺入模式的分析表明，在最初的大约 20 小时的“应激反应”期内，呼吸型代谢增加，光合作用减少。在有利的条件下，这个时期之后是快速恢复，以及重新建立一种代谢状态，这种状态与完整叶片的代谢状态惊人地相似，其中 CO2 的初始固定速率为每毫克叶绿素每小时 110 到 175 微摩尔 CO2。这些细胞的光合活力可以维持长达 80 小时。
