Department of Agricultural Biochemistry, School of Agriculture, University of Edinburgh, West Mains Road, Edinburgh EH9 3JG Scotland.
Plant Physiol. 1978 Sep;62(3):360-2. doi: 10.1104/pp.62.3.360.
Rates of oxygen exchange in light and dark were recorded for immature detached barley spikelets and wheat florets both before and after successive removal of the husk (palea and lemma), transparent layer of the pericarp, and green layer of the pericarp. Results were compared with those for the mutant barley Albino lemma which has a pericarp lacking chlorophyll. There was no net oxygen evolution in the intact spikelets of Albino lemma when incubated in the light. Removal of the husk increased the rate of measured oxygen uptake in both light and dark. With normal barley and wheat, net oxygen evolution in the light was observed in intact spikelets and florets, as well as after husk removal and after both husk and transparent layer removal. Additional removal of the green layer of the pericarp resulted in a dramatic changeover from oxygen evolution in the light to oxygen uptake. The results suggest that some of the oxygen generated by pericarp photosynthesis remains within the grain.
记录了去壳（内稃和外稃）、透明果皮层和绿色果皮层后，不成熟的大麦小穗和小麦小花在光下和暗中的氧气交换速率。将结果与缺乏叶绿素的突变体大麦白化外稃进行了比较。在光照下，完整的白化外稃小穗中没有净氧气产生。去壳增加了光和暗条件下测量的氧气吸收速率。对于正常的大麦和小麦，在完整的小穗和小花中以及去壳后和去壳及透明层后，都观察到了在光下的净氧气释放。进一步去除果皮的绿色层会导致光合作用产生的氧气从光下释放转变为氧气吸收。结果表明，果皮光合作用产生的部分氧气仍留在谷物内。
