Department of Biochemistry, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78284.
Plant Physiol. 1978 Sep;62(3):373-6. doi: 10.1104/pp.62.3.373.
The particulate enzyme fraction from mung bean (Phaseolus aureus) seedlings catalyzes the incorporation of mannose from GDP-[(14)C]mannose into mannosyl-phosphoryl-dolichol and of N-acetylglucosamine from UDP-[(3)H]N-acetylglucosamine into N-acetylglucosamine-pyrophosphoryl-polyisoprenol. Bacitracin inhibits the transfer of both of these sugars into the lipid-linked saccharides with 50% inhibition being observed at 5 mm bacitracin. This antibiotic did not inhibit the transfer of glucose from UDP-[(14)C]glucose into steryl glucosides or the incorporation of glucose into a cell wall glucan. Bacitracin also inhibited the in vivo incorporation of [(14)C]mannose into mannosyl-phosphoryl-dolichol and into glycoprotein by carrot (Daucus carota) slices. While bacitracin also inhibited the incorporation of lysine into proteins by these slices, protein synthesis was less sensitive than glycosylation. Thus at 2 mm bacitracin glycosylation was inhibited 92%, while protein synthesis was inhibited only 50%.
绿豆（Phaseolus aureus）幼苗的颗粒酶部分将 GDP-[(14)C]甘露糖中的甘露糖和 UDP-[(3)H]N-乙酰葡萄糖胺中的 N-乙酰葡萄糖胺掺入到甘露糖磷酸多萜醇和 N-乙酰葡萄糖胺焦磷酸多萜醇中。杆菌肽抑制这两种糖向脂连接的糖基转移，5 mM 杆菌肽时抑制率为 50%。该抗生素不抑制 UDP-[(14)C]葡萄糖向甾醇葡糖苷的葡萄糖转移，也不抑制葡萄糖掺入细胞壁葡聚糖。杆菌肽还抑制胡萝卜（Daucus carota）切片中 [(14)C]甘露糖掺入甘露糖磷酸多萜醇和糖蛋白。虽然杆菌肽也抑制这些切片中赖氨酸掺入蛋白质，但蛋白质合成的敏感性低于糖基化。因此，在 2 mM 杆菌肽时，糖基化抑制 92%，而蛋白质合成仅抑制 50%。
